AOAC CASP Meeting - MYM 2020

Method Performance Requirements

Table 3. Acceptable Matrix Claims

Table 2. Category Test Portion Requirements

Minimum Test Portion Size a

Criteria

Category

Matrix Claim

Plants & Flowers

10 g

Number of Matrices Minimum Number of Categories

15 (minimum 3 matrices/category) ≥ 10 (minimum 2 matrices/category)

Concentrates

5 g

Broad Range

4 categories

Infused Edibles

25 g

Variety

4 categories 2 categories 1 category

Infused Non ‐ Edibles 10 g a Minimum test portion size required for validation. Alternatively, larger test portions may be validated.

Select

≥ 5 ≥ 5 ≥ 1

Specific Category Specific Matrix (s)

1 category RE: AOAC Technical Bulletin: TB02MAY2016: Acceptable Validation Claims for Proprietary/Commercial Microbiology Methods for Foods and Environmental Surfaces.

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Method Performance Requirements

Table 5. Condition of Inoculating Culture and Stabilization of Matrix Table 6: Recommended Secondary Selective Broths and Agar

Stabilization Conditions 4°C, 48 ‐ 72 h 4°C, 48 ‐ 72 h

Matrix

Inoculating Cells

Media Name

Media Type

Perishable product Heat processed perishable product

Liquid non ‐ stressed culture

Liquid heat stressed

Rappaport ‐ Vassiliadis (RV) (alternately Rappaport ‐ Vassiliadis R10)

Broth

Liquid non ‐ stressed culture (If frozen food is processed, cells must be heat stressed)

‐ 20°C, 2 weeks

Broth Broth

Tetrathionate (TT)

Frozen Product

Selenite cysteine (SC)

Ambient Temperature (20 ‐ 25 o C), 2 weeks Ambient Temperature (20 ‐ 25 o C), 2 weeks

Agar Agar Agar Agar Agar

Xylose lysine desoxycholate (XLD)

Dried culture

Hektoen enteric (HE) Bismuth sulfite (BS) Chromogenic Salmonella

Shelf stable dry product

Liquid non ‐ stressed culture (If shelf stable product is processed, cells must be heat stressed)

Shelf stable liquid product (heat processed)

MacConkey

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