AOAC CASP Meeting - MYM 2020

Pre-decisional draft, do not distribute

Table 5. Condition of Inoculating Culture and Stabilization of Matrix

Stabilization Conditions 4°C, 48-72 h 4°C, 48-72 h

Matrix

Inoculating Cells

Liquid non-stressed culture

Perishable product Heat processed perishable product

Liquid heat stressed

Liquid non-stressed culture (If frozen food is processed, cells must be heat stressed)

-20°C, 2 weeks

Frozen Product

Ambient Temperature (20-25 o C), 2 weeks Ambient Temperature (20-25 o C), 2 weeks

Dried culture

Shelf stable dry product

Liquid non-stressed culture (If shelf stable product is processed, cells must be heat stressed)

Shelf stable liquid product (heat processed)

Table 6: Recommended Secondary Selective Broths and Agar

Media Type

Media Name

Rappaport-Vassiliadis (RV) (alternately Rappaport-Vassiliadis R10)

Broth Broth Broth Agar Agar Agar Agar Agar

Tetrathionate (TT) Selenite cysteine (SC)

Xylose lysine desoxycholate (XLD)

Hektoen enteric (HE) Bismuth sulfite (BS) Chromogenic Salmonella

MacConkey

8

Draft Salmonella SMPR v6