AOAC CASP Meeting

Method Performance Requirements

Table 2. Category Test Portion Requirements

Table 3. Acceptable Matrix Claims

Minimum Test  Portion Size a

Criteria

Category

Matrix Claim

Plants & Flowers

10 g 

Number of Matrices Minimum Number  of Categories

15 (minimum 3  matrices/category) ≥ 10 (minimum 2  matrices/category)

Concentrates

5 g

Broad Range

4 categories

Infused Edibles 

25 g

Variety

4 categories 2 categories 1 category 1 category

Infused Non‐Edibles 10 g a Minimum test portion size required for validation.   Alternatively, larger test portions may be validated.  

Select

≥ 5 ≥ 5 ≥ 1

Specific Category Specific Matrix (s)

Comments Submitted

The definition for acceptable minimum detection level does not align  with the final version presented in ISO 16140‐1. No minimum detection  level has been set as a requirement; therefore this definition has been  removed from the document.  Table 1 has been modified to emphasize  the low contamination level should produce fractional positive results.  This aligns the SMPR with Appendix J.    After spores, more descriptive language regarding the state of the  inoculum (liquid stressed/non‐stressed, lyophilized) has been added  parenthetically. The SMPR  has been updated to the following: At the time of the  publication, no national reference standard method exists for the  confirmation of Aspergillus from cannabis products.  The SMPR  has been updated to: Until a suitable reference method is  established the following is recommended for method developers. The SMPR has been updated to change probably to probable.

Line 34: The entire 90% confidence interval  on the POD falls above 0.95. 

Line 154: Add additional details regarding  condition of culture for inoculation Line 165:  Suggestion to replace word  "standard" with "method" Line 166:  Replace "until one" with "until a  suitable reference standard method ... Line 190:  "probable" not "probably"