AOAC ERP Gluten Assays - March 2019

, 00(5 + $$6 / $87(5%$&+ - 2851$/ 2) $2$& , 17(51$7,21$/ 9 2/

1 2

,QWHUODERUDWRU\ VWXG\ UHVXOWV IRU JOLDGLQ E\ 5,'$6&5((1 Š *OLDGLQ 0DWHULDO

7DEOH

1R RI ODEV RXWOLHUV

5HSHDWDELOLW\ 56' U

5HSURGXFLELOLW\ 56' 5

0DWUL[

/HYHO PJ NJ

0HDQ PJ NJ 5HFRYHU\

0DL]H 0DL]H 0DL]H 0DL]H 5LFH 5LFH D 5LFH :KHDW VWDUFK 5LFH ÀRXU :KHDW VWDUFK

0DL]H ÀRXU D 0DL]H ÀRXU D D 1HJDWLYH VDPSOHV ZHUH QRW LQFOXGHG LQ WKH VWDWLVWLFDO HYDOXDWLRQ VHH 5HVXOWV DQG 'LVFXVVLRQ

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¿HG KHUH KDV EHHQ WHVWHG (TXLYDOHQW DSSDUDWXV PD\ EH XVHG D *ULQGRPL[ *0 ²)RU VDPSOH KRPRJHQL]DWLRQ 5HWVFK *PE+ +DDU *HUPDQ\ E :DWHU EDWK ²*)/ *HV I /DERUWHFKQLN PE+ %XUJZHGHO *HUPDQ\ F %HQFK WRS FHQWULIXJH ²0XOWLIXJH / 5 RSHUDWLQJ DW USP 7KHUPR (OHFWURQ *PE+ 'UHLHLFK *HUPDQ\ G *ODVV WXEHV P/ ² )RU H[WUDFWLRQ %UDQG *PE+ :HUWKHLP *HUPDQ\ H 3RO\VW\URO WXEHV P/ ²)RU VDPSOH GLOXWLRQ 6DUVWHGW 1PEUHFKW *HUPDQ\ I 0LFURWLWHU SODWH UHDGHU ZLWK QP ¿OWHU ²7HFDQ 'HXWVFKODQG *PE+ &UDLOVKHLP *HUPDQ\ J 0LFURSLSHW ²$FFXUDWHO\ GHOLYHULQJ —/ “ K *ODVVZDUH ²:DVK ERWWOH P/ JUDGXDWHG F\OLQGHUV L 5RWDWRU &09 RU HTXLYDOHQW ²)U|EHO /DERUWHFKQLN /LQGDX *HUPDQ\ % $SSDUDWXV

%LQG WR JOLDGLQ GHULYHG IURP ZKHDW DQG WR UHODWHG

SURODPLQV GHULYHG IURP U\H DQG EDUOH\

5HFRJQL]H WKH SRWHQWLDO FHOLDF WR[LF VWUXFWXUH 443)3

DQG UHODWHG VHTXHQFHV

%LQG WR WKH DOSKD EHWD JDPPD DQG RPHJD JOLDGLQ PRWLIV LQ QRQKHDWHG DQG KHDWHG IRRG H[WUDFWHG E\ FRFNWDLO VROXWLRQ 1R ELQGLQJ WR RDWV PDL]H ULFH WHII EXFNZKHDW TXLQRD DQG DPDUDQWK %LQG ZLWK KLJK DI¿QLW\ WR DOORZ DQ /2' RI PJ NJ JOLDGLQ RU UHODWHG SURODPLQV $EOH WR EXLOG D VWDEOH 32' ODEHOHG FRQMXJDWH VWDEOH IRU PRUH WKDQ \HDU 6KRZ UHSURGXFLEOH DI¿QLW\ VHQVLWLYLW\ VSHFL¿FLW\ DQG VWDELOLW\ IURP EDWFK WR EDWFK IRU PRUH WKDQ \HDU 0RQRFORQDO DQWLERGLHV DUH SUHIHUUHG SRO\FORQDO DQWLERGLHV FDQ EH XVHG LI WKH\ IXO¿O WKH VDPH VSHFL¿FLW\ FULWHULD WR UHDFW ZLWK ZKHDW U\H DQG EDUOH\ WR DQG KDYH QR FURVV UHDFWLYLW\ WR RDW PDL]H WHII DQG RWKHUV ,WHPV D ± L DUH DYDLODEOH DV D WHVW NLW 5 %LRSKDUP $* $OO UHDJHQWV DUH VWDEOH IRU PRQWKV IURP GDWH RI PDQXIDFWXUH DW ± ƒ& ± ƒ) 5HIHU WR NLW ODEHO IRU FXUUHQW H[SLUDWLRQ (TXLYDOHQW DQWLERGLHV PD\ EH XVHG IRU D DQG F SURYLGHG WKH\ VDWLVI\ FKDUDFWHULVWLF FULWHULD GHVFULEHG LQ & DERYH D $QWLERG\ FRDWHG PLFURZHOO VWULSV ²0RQRFORQDO DQWLERGLHV DUH FRDWHG LQ P0 SKRVSKDWH EXIIHUHG VDOLQH 3%6 S+ RQWR D VHW RI WZHOYH PLFURZHOO VWULSV 181& 5RVNLOGH 'HQPDUN FRQWDLQLQJ VRGLXP D]LGH DV SUHVHUYDWLYH E :DVK EXIIHU FRQFHQWUDWH P/ ERWWOH [ FRQFHQWUDWH ² &RQWDLQV D ¿QDO FRQFHQWUDWLRQ RI P0 3%6 VRGLXP FKORULGH ZLWK 6\QSHURQLF DQG .DWKRQ DV SUHVHUYDWLYH ' 5HDJHQWV

& $QWLERG\ &KDUDFWHULVWLFV

$QWLERGLHV PXVW VDWLVI\ WKH IROORZLQJ FULWHULD