AOAC ERP MICRO AUGUST 2018

OMAMAN-44 A: Collaborative Study Manuscript Expert Review Panel Use Only August 2018

Pre-collaborative and Collaborative Report Peel Plate EB

Table 4. Peel Plate-EB Method (Singlet count) for Enterobacteriaceae at 24 hours Compared to ISO Methods 21528-1 and 2

Fortified micro- organisms ATCC No. Enterobacter Amnigenus ATCC 1 51816 (Heat Stressed)

Candidate Method

Reference Method

95% CI e

Cont. level

Mean diff. d

r 2 h

Matrix

Mean a

s r

RSD r

Mean a

s r

RSD r

LCL f

UCL g

b

c

b

c

None

<0.1

na

na 22

<0.1

na

na 28

na

na

na

3.25% past. whole milk j

Low

1.032 3.453 4.177 2.245 3.205 4.877 1.768 3.637 4.842 2.205 3.153 4.946 1.658 3.240 4.636 1.177 2.477 3.593 <0.1 <0.1 <0.1 <0.1

0.233 0.153 0.116 0.114 0.084 0.101 0.137 0.056 0.099 0.084 0.126 0.120 0.073 0.059 0.026 0.090 0.036 0.059 na na na na

1.017 3.429 4.199 2.216 3.203 4.889 1.669 3.612 4.835 2.216 3.203 4.889 1.626 3.251 4.626 1.152 2.469 3.563 <0.1 <0.1 <0.1 <0.1

0.289 0.087 0.144 0.146 0.102 0.150 0.232 0.064 0.067 0.146 0.102 0.150 0.089 0.085 0.053 0.105 0.027 0.048 na na na na

0.015 0.024 -0.022 0.029 0.003 -0.12 0.099 0.254 0.008 -0.011 -0.050 0.057 0.059 -0.012 0.010 0.026 0.008 0.031 na na na na

-0.198 -0.106 -0.130 -0.030 -0.078 -0.131 -0.057 -0.028 -0.062 -0.101 -0.172 -0.126 -0.011 -0.122 -0.057 -0.014 -0.033 -0.079 na na na na

0.227 0.154 0.086 0.088 0.083 0.107 0.255 0.079 0.078 0.079 0.073 0.240 0.128 0.099 0.077 0.068 0.050 0.140 na na na na

0.993

Medium

4 3

3 3

High None

na

na

Hafnia alvei ATCC 51815 (Lyophilized)

Non-fat dry milk powder j

Low

5 3 2 8 2 2 4 4 2 4 2 1 8 1 2

7 3 3

0.909

Medium

High None

na

na 14

Cronobacter sakazakii CCUG 2 28863 (Lyophilized) Escherichia coli ATCC 25922 (Lyophilized)

Infant Formula with probiotic j Infant Cereal with Probiotic j Sponge sample from Stainless Steel Chicken Rinse in n- BPW

Low

0.997

Medium

2 1

High None

na

na

Low

7 3 3 5 3 1 9 1 1

0.992

Medium

High None

na

na

Salmonella Typhimurium ATCC 14028

Low

0.997

Medium

High ERP Use Only Natural Medium High

0.997

Natural Contamination

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