AOAC ERP MICRO AUGUST 2018

OMAMAN-44 A: Collaborative Study Manuscript Expert Review Panel Use Only August 2018

Pre-collaborative and Collaborative Report Peel Plate EB

Table 6. Peel Plate-EB Method (Singlet count) for Enterobacteriaceae at 48 hours Compared to ISO Methods 21528-1 and 2.

Fortified micro- organisms ATCC No. Enterobacter Amnigenus ATCC 51816 (Heat Stressed) Hafnia alvei ATCC 51815 (Lyophilized) Cronobacter sakazakii CCUG 2 28863 (Lyophilized) Escherichia coli ATCC 25922 (Lyophilized)

Candidate Method

Reference Method

95% CI e

Cont. level

Mean diff. d

r 2 h

Matrix

Mean a 1.036 3.465 4.183 1.890 3.559 3.991 1.768 3.658 4.862 2.211 3.158 4.956 1.172 3.255 4.640 1.036 1.191 2.486 3.598 <0.1 <0.1 <0.1 <0.1

s r

RSD r

Mean a

s r

RSD r

LCL f

UCL g 0.229 0.168 0.101 0.081 0.161 0.296 0.281 0.104 0.111 0.086 0.077 0.271 0.243 0.122 0.079 0.229 0.079 0.056 0.137 na na na na

b

c

b

c

1.017 3.429 4.199 1.896 3.504 4.892 1.669 3.612 4.835 2.216 3.203 4.889 1.626 3.251 4.626 <0.1 <0.1 <0.1 <0.1

Low

0.239 0.151 0.117 0.186 0.094 0.147 0.137 0.063 0.122 0.080 0.130 0.118 0.071 0.059 0.026 0.239 0.077 0.036 0.053 na na na na

23

0.289 0.087 0.144 0.204 0.106 0.081 0.232 0.064 0.067 0.146 0.102 0.150 0.089 0.085 0.053 0.289 0.105 0.027 0.048 na na na na

28

0.019 0.036 -0.016 -0.006 0.068 0.100 0.110 0.046 0.027 -0.005 -0.045 0.067 0.093 0.003 0.014 0.019 0.039 0.017 0.036 na na na na

-0.191 -0.096 -0.133 -0.092 -0.024 -0.096 -0.060 -0.012 -0.058 -0.096 -0.166 -0.137 -0.056 -0.116 -0.051 -0.191 -0.002 -0.023 -0.065 na na na na

3.25% past. whole milk j

Medium

4 3

3 3

0.993

High None

na 10

na 11

Low

Non-fat dry milk powder j Infant Formula with probiotic j Infant Cereal with Probiotic j Sponge sample from Stainless Steel Chicken Rinse in n-BPW

Medium

3 4

3 2

0.913

High None

na

na 14

Low

8 2 3 4 4 2 4 2 1

Medium

2 1

0.997

High None

na

na

Low

7 3 3 5 3 1

Medium

0.990

High None

na

na

Low

Salmonella Typhimurium ATCC 14028

Medium

0.997

High

Low Low ERP Use Only 23 1.017 28 6 1 1 1.152 2.469 3.563 9 1 1 Medium High

Natural Contamination

0.997

38