AOAC ERP MICRO AUGUST 2018
OMAMAN-44 A: Collaborative Study Manuscript Expert Review Panel Use Only August 2018
Pre-collaborative and Collaborative Report Peel Plate EB
Table 8. Peel Plate-EB High Volume (HV) Method (Singlet count) for Enterobacteriaceae at 24 hours vs ISO Methods 21528-1 & 2.
Fortified micro- organisms
Reference Method
r 2 h
Candidate Method
95% CI e
Mean diff. d
Matrix
Cont. level
ATCC No. (%injury)
Mean a
s r b
RSD r c
Mean
s r
RSD r
LCL f
UCL g
None
<0.1
na
na
<0.1
na
na
na
na
na
Infant Cereal with Probiotic j
Escherichia coli ATCC 25922 (Lyophilized)
Low
2.228 3.199 4.852 1.586 3.838 4.819 <0.1
0.125 0.088 0.110 0.354 0.023 0.108 na
6 3 2
2.216 3.203 4.889 1.551 3.559 4.592 <0.1
0.146 0.102 0.150 0.154 0.139 0.083 na
7 3 3
0.014 -0.004 -0.037 0.034 0.279 0.227 na
-0.049 -0.077 -0.114 -0.388 0.128 0.115 na
0.073 0.070 0.064 0.457 0.429 0.339 na
0.997
Medium
High None
na 10
na 22
Citrobacter freundii ATCC 8090 (Lyophilized)
Rice Infant cereal
low mid high
0.998
1 2
4 2
Table 8 continued. Peel Plate-EBHV Method (Duplicate count) for Enterobacteriaceae at 48 hours vs ISO Methods 21528-1 and 2.
Fortified micro- organisms
Reference Method
r 2 h
Candidate Method
95% CI e
Mean diff. d
Matrix
Cont. level
ATCC No. (%injury)
Mean a
s r b
RSD r c
Mean
s r
RSD r
LCL f
UCL g
None
<0.1
na
na
<0.1
na
na
na
na
na
Infant Cereal with Probiotic j
Escherichia coli ATCC 25922 (Lyophilized)
low mid high
2.245 3.205 4.877 1.561 3.830 4.855 <0.1
0.114 0.084 0.101 0.356 0.043 0.103 na
5 3 2
2.216 3.203 4.889 1.551 3.563 4.592 <0.1
0.146 0.102 0.150 0.154 0.133 0.083 na
7 3 3
0.029 0.002 -0.012 0.010 0.267 0.263 na
-0.030 -0.078 -0.131 -0.438 0.113 0.160 na
0.088 0.083 0.107 0.459 0.422 0.365 na
0.997
None
na 23
na 10
Citrobacter freundii ATCC 8090 (Lyophilized)
low mid high ERP Use Only 1 2 4 2
Rice Infant cereal
0.98
a Mean of 5 replicate portions, plated in duplicate, after logarithmic transformation: Log 10 [CFU/g + (0.1)f]. b Repeatability standard deviation.
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