AOAC ERP MICRO AUGUST 2018

OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016

Table 8: 3M ™ MDA 2 - Salmonella Assay, Candidate vs. Reference – POD Results

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD C

x c

d 95% CI

e

POD C

X

POD R

95% CI

-

5

0

0.00 0.55 1.00 0.00 0.55 1.00

0.00, 0.43 0.34, 0.74 0.57, 1.00 0.00, 0.43 0.34, 0.74 0.57, 1.00

0 9 5 0 9 5

0.00 0.45 1.00 0.00 0.45 1.00

0.00, 0.43 0.00 0.26, 0.66 0.10 0.57, 1.00 0.00 0.00, 0.43 0.00 0.26, 0.66 0.10 0.57, 1.00 0.00

-0.43, 0.43 -0.19, 0.37 -0.43, 0.43 -0.43, 0.43 -0.19, 0.37 -0.43, 0.43

10 Hours

0.75 (0.44, 1.23) 20

11

Raw Ground Beef (73% Lean) (25 g)

4.38 (1.72, 11.15)

5

5

Salmonella Agona ATCC 51957

-

5

0

24 Hours

0.75 (0.44, 1.23) 20

11

4.38 (1.72, 11.15)

5

5

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD C

x c

d 95% CI

e

POD C

X

POD R

95% CI

-

5

0 7

0.00 0.35

0.00, 0.43 0.18, 0.57

0 9

0.00 0.45

0.00, 0.43 0.00 0.26, 0.66 -0.10

-0.43, 0.43 -0.37, 0.19

10 Hours

0.75 (0.44, 1.23) 20

Raw Ground Beef (73% Lean) (325 g)

4.38 (1.72, 11.15)

Salmonella Agona ATCC 51957

5

5

1.00

0.57, 1.00

5

1.00

0.57, 1.00 0.00

-0.43, 0.43

-

5

0 7 5

0.00 0.35 1.00

0.00, 0.43 0.18, 0.57 0.57, 1.00

0 9 5

0.00 0.45 1.00

0.00, 0.43 0.00 0.26, 0.66 -0.10 0.57, 1.00 0.00

-0.43, 0.43 -0.37, 0.19 -0.43, 0.43

24 Hours

0.75 (0.44, 1.23) 20

4.38 (1.72, 11.15)

5

a MPN = Most Probable Number is calculated using the LCF MPN calculator provided by AOAC RI, with 95% confidence interval b N = Number of test portions c x = Number of positive test portions d POD C = Candidate method confirmed positive outcomes divided by the total number of trials e POD R = Reference method confirmed positive outcomes divided by the total number of trials f dPOD C = Difference between the confirmed candidate method result and reference method confirmed result POD values g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level AOAC Research Institute

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