AOAC ERP MICRO AUGUST 2018
OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016
Table 9: 3M ™ MDA 2 - Salmonella Assay, Candidate vs. Reference – POD Results
Analysis Time Point
Candidate
Reference
MPN a / Test Portion
Matrix/Test Portion
N b
Strain
dPODCf 95% CIg
x c
d 95% CI
e
POD C
X
POD R
95% CI
-
5
0 9 5 0 9 5 0 8 5 0 8 5
0.00 0.45 1.00 0.00 0.45 1.00 0.00 0.40 1.00 0.00 0.40 1.00
0.00, 0.43 0.26, 0.66 0.57, 1.00 0.00, 0.43 0.26, 0.66 0.57, 1.00 0.00, 0.43 0.22, 0.61 0.57, 1.00 0.00, 0.43 0.22, 0.61 0.57, 1.00
0 9 5 0 9 5 0 9 5 0 9 5
0.00 0.45 1.00 0.00 0.45 1.00 0.00 0.45 1.00 0.00 0.45 1.00
0.00, 0.43 0.00 0.26, 0.66 0.00 0.57, 1.00 0.00 0.00, 0.43 0.00 0.26, 0.66 0.00 0.57, 1.00 0.00
-0.43, 0.43 -0.28, 0.28 -0.43, 0.43 -0.43, 0.43 -0.28, 0.28 -0.43, 0.43 -0.43, 0.43 -0.33, 0.24 -0.43, 0.43 -0.43, 0.43 -0.33, 0.24 -0.43, 0.43
10 Hours
0.56 (0.29, 0.94) 20
4.38 (1.72, 11.15)
Raw Ground Chicken (25 g)
5
Salmonella Heidelberg NCTC 5717
-
5
24 Hours
0.56 (0.29, 0.94) 20
4.38 (1.72, 11.15)
5
Analysis Time Point
Candidate
Reference
MPN a / Test Portion
Matrix/Test Portion
N b
f 95% CI g
Strain
dPOD C
x c
d 95% CI
e
POD C
X
POD R
95% CI
-
5
0.00, 0.43 0.00 0.26, 0.66 -0.05
14 Hours
0.56 (0.29, 0.94) 20
4.38 (1.72, 11.15)
Raw Ground Chicken (325 g)
5
0.57, 1.00 0.00
Salmonella Heidelberg NCTC 5717
-
5
0.00, 0.43 0.00 0.26, 0.66 -0.05 0.57, 1.00 0.00
24 Hours
0.56 (0.29, 0.94) 20
4.38 (1.72, 11.15)
5
a MPN = Most Probable Number is calculated using the LCF MPN calculator provided by AOAC RI, with 95% confidence interval b N = Number of test portions c x = Number of positive test portions d POD C = Candidate method confirmed positive outcomes divided by the total number of trials e POD R = Reference method confirmed positive outcomes divided by the total number of trials f dPOD C = Difference between the confirmed candidate method result and reference method confirmed result POD values g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level AOAC Research Institute
Expert Review Panel Use Only
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