AOAC ERP MICRO AUGUST 2018

OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016

Table 11: 3M ™ MDA 2 - Salmonella Assay, Candidate vs. Reference – POD Results

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

Matrix/Test Portion Instant Non- fat Dry Milk (25 g) Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD C

x c

d 95% CI

e

POD C

X

POD R

95% CI

-

5

0 7 5

0.00 0.35 1.00

0.00, 0.43 0.18, 0.57 0.57, 1.00

0 7 5

0.00 0.35 1.00

0.00, 0.43 0.00 0.18, 0.57 0.00 0.57, 1.00 0.00

-0.43, 0.43 -0.28, 0.28 -0.43, 0.43

Salmonella Infantis ATCC 51741

20 Hours

0.54 (0.29, 0.91) 20 3.01 (1.31, 6.89) 5

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

N b

f 95% CI g

Strain

dPOD C

x c

d 95% CI

e

POD C

X

POD R

95% CI

-

5

0 8 5

0.00 0.40 1.00

0.00, 0.43 0.22, 0.61 0.57, 1.00

0 5 5

0.00 0.25 1.00

0.00, 0.43 0.00 0.11, 0.47 0.15 0.57, 1.00 0.00

-0.43, 0.43 -0.13, 0.40 -0.43, 0.43

Black Pepper (25 g)

Salmonella Tallahassee ATCC 12002

18 Hours

0.35 (0.16, 0.63) 20 3.01 (1.31, 6.89) 5

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD C

x c

d 95% CI

e

POD C

X

POD R

95% CI

-

5

0 8 5

0.00 0.40 1.00

0.00, 0.43 0.22, 0.61 0.57, 1.00

0 8 5

0.00 0.40 1.00

0.00, 0.43 0.00 0.22, 0.61 0.00 0.57, 1.00 0.00

-0.43, 0.43 -0.28, 0.28 -0.43, 0.43

Cocoa Powder (25 g)

Salmonella Newport ATCC 6962

24 Hours

0.55 (0.29, 0.92) 20 2.29 (1.04, 5.02) 5

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

Matrix/Test Portion Pasteurized Liquid Whole Egg (100 g)

N b

f 95% CI g

Strain

dPOD C

x c

d 95% CI

e

POD C

X

POD R

95% CI

-

5

0

0.00 0.55 1.00

0.00, 0.43 0.34, 0.74 0.57, 1.00

0 9 5

0.00 0.45 1.00

0.00, 0.43 0.00 0.26, 0.66 0.10 0.57, 1.00 0.00

-0.43, 0.43 -0.19, 0.37 -0.43, 0.43

Salmonella Enteritidis ATCC 10376

18 Hours

0.68 (0.39, 1.12) 20 2.62 (1.20, 5.74) 5

11

5 a MPN = Most Probable Number is calculated using the LCF MPN calculator provided by AOAC RI, with 95% confidence interval b N = Number of test portions c x = Number of positive test portions d POD C = Candidate method confirmed positive outcomes divided by the total number of trials e POD R = Reference method confirmed positive outcomes divided by the total number of trials f dPOD C = Difference between the confirmed candidate method result and reference method confirmed result POD values g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level AOAC Research Institute

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