AOAC ERP MICRO AUGUST 2018

OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016

Table 12: 3M ™ MDA 2 - Salmonella Assay, Candidate vs. Reference – POD Results

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD C

x c

d 95% CI

e

POD C

X

POD R

95% CI

-

5

0

0.00 0.50 1.00

0.00, 0.43 0.30, 0.70 0.57, 1.00

0

0.00 0.60 1.00

0.00, 0.43 0.00 0.39, 0.78 -0.10

-0.43, 0.43 -0.37, 0.19 -0.43, 0.43

Raw Whole Shrimp (25 g)

Salmonella Virchow ATCC 51955

18 Hours

0.99 (0.60, 1.69) 20

10

12

4.38 (1.72, 11.15)

5

5

5

0.57, 1.00 0.00

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD C

x c

d 95% CI

e

POD C

X

POD R

95% CI

-

5

0 6 5

0.00 0.30 1.00

0.00, 0.43 0.15, 0.52 0.57, 1.00

0 5 5

0.00 0.25 1.00

0.00, 0.43 0.00 0.11, 0.47 0.05 0.57, 1.00 0.00

-0.43, 0.43 -0.22, 0.31 -0.43, 0.43

Raw Bagged Spinach (25 g)

Salmonella Schwarzengrund ATCC STs 95

18 Hours

0.35 (0.17, 0.63) 20 3.01 (1.31, 6.89) 5

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD C

x c

d 95% CI

e

POD C

X

POD R

95% CI

Spent Sprout Irrigation Water (375 mL)

-

5

0

0.00 0.55

0.00, 0.43 0.34, 0.74

0 9

0.00 0.45

0.00, 0.43 0.00 0.26, 0.66 0.10

-0.43, 0.43 -0.19, 0.37

Salmonella Saintpaul ATCC 9712

0.68 (0.39, 1.12) 20

11

18 Hours

3.82 (1.57, 9.32) 5

5

1.00

0.57, 1.00

5

1.00

0.57, 1.00 0.00

-0.43, 0.43

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD C

x c

d 95% CI

e

POD C

X

POD R

95% CI

-

5

0 8 5

0.00 0.40 1.00

0.00, 0.43 0.22, 0.61 0.57, 1.00

0 6 5

0.00 0.30 1.00

0.00, 0.43 0.00 0.15, 0.52 0.10 0.57, 1.00 0.00

-0.43, 0.43 -0.18, 0.36 -0.43, 0.43

Cooked Breaded Chicken (325 g)

Salmonella Kentucky ATCC 9263 a MPN = Most Probable Number is calculated using the LCF MPN calculator provided by AOAC RI, with 95% confidence interval b N = Number of test portions c x = Number of positive test portions d POD C = Candidate method confirmed positive outcomes divided by the total number of trials e POD R = Reference method confirmed positive outcomes divided by the total number of trials f dPOD C = Difference between the confirmed candidate method result and reference method confirmed result POD values g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level AOAC Research Institute 18 Hours 0.44 (0.21, 0.76) 20 2.64 (1.20, 5.81) 5

Expert Review Panel Use Only