AOAC ERP MICRO AUGUST 2018

OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016

Table 13: 3M ™ MDA 2 - Salmonella Assay, Candidate vs. Reference – POD Results

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD C

x c

d 95% CI

e

POD C

X

POD R

95% CI

-

5

0

0.00 0.55 1.00

0.00, 0.43 0.34, 0.74 0.57, 1.00

0 8 5

0.00 0.40 1.00

0.00, 0.43 0.00 0.22, 0.61 0.15 0.57, 1.00 0.00

-0.43, 0.43 -0.15, 0.41 -0.43, 0.43

Creamy Peanut Butter (25 g)

Salmonella Muenchen ATCC BAA 1594

18 Hours

0.55 (0.29, 0.93) 20 3.01 (1.31, 6.89) 5

11

5

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD C

x c

d 95% CI

e

POD C

X

POD R

95% CI

-

5

0 5 5

0.00 0.25 1.00

0.00, 0.43 0.11, 0.47 0.57, 1.00

0 8 5

0.00 0.40 1.00

0.00, 0.43 0.00

-0.43, 0.43

Creamy Peanut Butter (375 g)

Salmonella Muenchen ATCC BAA 1594

18 Hours

0.55 (0.29, 0.93) 20 3.01 (1.31, 6.89) 5

0.22, 0.61 -0.15 -0.40, 0.13

0.57, 1.00 0.00

-0.43, 0.43

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD C

x c

d 95% CI

e

POD C

X

POD R

95% CI

-

5

0

0.00 0.55 1.00

0.00, 0.43 0.34, 0.74 0.57, 1.00

0

0.00 0.50 1.00

0.00, 0.43 0.00 0.30, 0.70 0.05 0.57, 1.00 0.00

-0.43, 0.43 -0.24, 0.33 -0.43, 0.43

Dry Dog Food (25 g)

Salmonella Typhimurium ATCC 14028

18 Hours

0.76 (0.44, 1.26) 20

11

10

4.38 (1.72, 11.15)

5

5

5

Analysis Time Point

Candidate

Reference

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD C

x c

d 95% CI

e

POD C

X

POD R

95% CI

-

5

0 7 5

0.00 0.35 1.00

0.00, 0.43 0.18, 0.57 0.57, 1.00

0

0.00 0.50 1.00

0.00, 0.43 0.00

-0.43, 0.43

Dry Dog Food (375 g)

Salmonella Typhimurium ATCC 14028

18 Hours a MPN = Most Probable Number is calculated using the LCF MPN calculator provided by AOAC RI, with 95% confidence interval b N = Number of test portions c x = Number of positive test portions d POD C = Candidate method confirmed positive outcomes divided by the total number of trials e POD R = Reference method confirmed positive outcomes divided by the total number of trials f dPOD C = Difference between the confirmed candidate method result and reference method confirmed result POD values g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level AOAC Research Institute 0.76 (0.44, 1.26) 20 10 4.38 (1.72, 11.15) 5 5

0.30, 0.70 -0.15 -0.41, 0.15

0.57, 1.00 0.00

-0.43, 0.43

Expert Review Panel Use Only