AOAC ERP MICRO AUGUST 2018
OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016
Table 15: 3M ™ MDA 2 - Salmonella Assay, Candidate vs. Reference – POD Results
Analysis Time Point
Candidate
Reference
CFU a / Test Area
N b
f 95% CI g
Matrix
Strain
dPOD C
x c
d 95% CI
e
POD C
X
POD R
95% CI
Salmonella Dublin STs 27 & Proteus vulgaris ATCC 6380
-
5
0
0.00 0.60
0.00, 0.43 0.39, 0.78
0
0.00 0.55
0.00, 0.43 0.00 0.34, 0.74 0.05
-0.43, 0.43 -0.24, 0.33
32 & 510
20
12
11
Sealed Concrete
18 Hours
400 & 3700
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00 0.00
-0.43, 0.43
Analysis Time Point
Candidate
Reference
CFU a / Test Area
N b
f 95% CI g
Matrix
Strain
dPOD C
x c
d 95% CI
e
POD C
X
POD R
95% CI
-
5
0
0.00 0.80 1.00
0.00, 0.43 0.58, 0.92 0.57, 1.00
0
0.00 0.65 1.00
0.00, 0.43 0.00 0.43, 0.82 0.15 0.57, 1.00 0.00
-0.43, 0.43 -0.12, 0.40 -0.43, 0.43
Stainless Steel
Salmonella Kahla ATCC 17980
18 Hours
57
20
16
13
510
5
5
5
Analysis Time Point
Candidate
Reference
CFU a / Test Area
N b
f 95% CI g
Matrix
Strain
dPOD C
x c
d 95% CI
e
POD C
X
POD R
95% CI
-
5
0 6 5
0.00 0.30 1.00
0.00, 0.43 0.15, 0.52 0.57, 1.00
0 9 5
0.00 0.45 1.00
0.00, 0.43 0.00 0.26, 0.66 -0.15 0.57, 1.00 0.00
-0.43, 0.43 -0.41, 0.14 -0.43, 0.43
Sealed ceramic Tile
Salmonella Anatum ATCC 9270
18 Hours
40
20
470
5
a CFU/Test Area = Results of the CFU/Test area were determined by plating the inoculum for each matrix in triplicate b N = Number of test portions c x = Number of positive test portions d POD C = Candidate method confirmed positive outcomes divided by the total number of trials e POD R = Reference method confirmed positive outcomes divided by the total number of trials f dPOD C = Difference between the confirmed candidate method result and reference method confirmed result POD values g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level AOAC Research Institute
Expert Review Panel Use Only
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