AOAC ERP MICRO AUGUST 2018

OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016

Table 16: 3M ™ MDA 2 - Salmonella Assay, Presumptive vs. Confirmed – POD Results

Analysi s Time Point

Presumptive

Confirmed

MPN a / Test Portion

Matrix/Tes t Portion

N b

f 95% CI g

Strain

dPOD CP

x c

d 95% CI

e 95% CI

POD CP

X

POD CC

-

5

0

0.00

0.00, 0.43 0

0.00

0.00, 0.43 0.00 0.34, 0.74 0.00 0.57, 1.00 0.00 0.00, 0.43 0.00 0.34, 0.74 0.00 0.57, 1.00 0.00 0.00, 0.43 0.00 0.18, 0.57 0.00 0.57, 1.00 0.00 0.00, 0.43 0.00 0.18, 0.57 0.00 0.57, 1.00 0.00

-0.43, 0.43 -0.28, 0.28 -0.43, 0.43 -0.43, 0.43 -0.28, 0.28 -0.43, 0.43 -0.43, 0.43 -0.28, 0.28 -0.43, 0.43 -0.43, 0.43 -0.28, 0.28 -0.43, 0.43

10 Hours

0.75 (0.44, 1.23) 20 11 0.55

0.34, 0.74 11 0.55

Raw Ground Beef (73% Lean) (25 g)

4.38 (1.72, 11.15)

5 5

5 0

1.00 0.00

0.57, 1.00 5 0.00, 0.43 0

1.00 0.00

Salmonella Agona ATCC 51957

-

24 Hours

0.75 (0.44, 1.23) 20 11 0.55

0.34, 0.74 11 0.55

4.38 (1.72, 11.15)

5

5

1.00

0.57, 1.00 5

1.00

Analysi s Time Point

Presumptive

Confirmed

MPN a / Test Portion

Matrix/Tes t Portion

N b

f 95% CI g

Strain

dPOD CP

x c

d 95% CI

e 95% CI

POD CP

X

POD CC

-

5

0

0.00 0.35 1.00 0.00 0.35 1.00

0.00, 0.43 0 0.18, 0.57 7 0.57, 1.00 5 0.00, 0.43 0 0.18, 0.57 7 0.57, 1.00 5

0.00 0.35 1.00 0.00 0.35 1.00

10 Hours

0.75 (0.44, 1.23) 20 7

Raw Ground Beef (73% Lean) (325 g)

4.38 (1.72, 11.15)

5 5

5 0

Salmonella Agona ATCC 51957

-

24 Hours

0.75 (0.44, 1.23) 20 7

4.38 (1.72, 11.15)

5

5

a MPN = Most Probable Number is calculated using the LCF MPN calculator provided by AOAC RI, with 95% confidence interval b N = Number of test portions c x = Number of positive test portions d POD CP = Candidate method presumptive positive outcomes divided by the total number of trials e POD CC = Candidate method confirmed positive outcomes divided by the total number of trials f dPOD CP = Difference between the candidate method presumptive result and candidate method confirmed result POD values g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level

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