AOAC ERP MICRO AUGUST 2018

OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016

Table 17: 3M ™ MDA 2 - Salmonella Assay, Presumptive vs. Confirmed – POD Results

Analysis Time Point

Presumptive x c POD CP

Confirmed

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD CP

d 95% CI

e 95% CI

X

POD CC

-

5

0

0.00 0.45 1.00 0.00 0.45 1.00

0.00, 0.43 0.26, 0.66 0.57, 1.00 0.00, 0.43 0.26, 0.66 0.57, 1.00 0.00, 0.43 0.30, 0.70 0.57, 1.00 0.00, 0.43 0.22, 0.61 0.57, 1.00

0 9 5 0 9 5 0 8 5 0 8 5

0.00 0.45 1.00 0.00 0.45 1.00 0.00 0.40 1.00 0.00 0.40 1.00

0.00, 0.43 0.00 0.26, 0.66 0.00 0.57, 1.00 0.00 0.00, 0.43 0.00 0.26, 0.66 0.00 0.57, 1.00 0.00 0.00, 0.43 0.00 0.22, 0.61 0.10 0.57, 1.00 0.00 0.00, 0.43 0.00 0.22, 0.61 0.00 0.57, 1.00 0.00

-0.43, 0.43 -0.28, 0.28 -0.43, 0.43 -0.43, 0.43 -0.28, 0.28 -0.43, 0.43 -0.43, 0.43 -0.19, 0.37 -0.43, 0.43 -0.43, 0.43 -0.28, 0.28 -0.43, 0.43

10 Hours

0.56 (0.29, 0.94) 20 9

4.38 (1.72, 11.15)

Raw Ground Chicken (25 g)

5 5

5 0

Salmonella Heidelberg NCTC 5717

-

24 Hours

0.56 (0.29, 0.94) 20 9

4.38 (1.72, 11.15)

5

5

Analysis Time Point

Presumptive x c POD CP

Confirmed

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD CP

d 95% CI

e 95% CI

X

POD CC

-

5

0

0.00

14 Hours

0.56 (0.29, 0.94) 20 10 0.50

4.38 (1.72, 11.15)

Raw Ground Chicken (325 g)

5 5

5 0

1.00 0.00 0.40 1.00

Salmonella Heidelberg NCTC 5717

-

24 Hours

0.56 (0.29, 0.94) 20 8

4.38 (1.72, 11.15)

5 AOAC Research Institute Expert Review Panel Use Only 5

a MPN = Most Probable Number is calculated using the LCF MPN calculator provided by AOAC RI, with 95% confidence interval b N = Number of test portions c x = Number of positive test portions d POD CP = Candidate method presumptive positive outcomes divided by the total number of trials e POD CC = Candidate method confirmed positive outcomes divided by the total number of trials f dPOD CP = Difference between the candidate method presumptive result and candidate method confirmed result POD values g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level