AOAC ERP MICRO AUGUST 2018
OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016
Table 19: 3M ™ MDA 2 Salmonella Assay, Presumptive vs. Confirmed – POD Results
Analysi s Time Point
Presumptive
Confirmed
MPN a / Test Portion
Matrix/Test Portion
N b
f 95% CI g
Strain
dPOD CP
x c
d 95% CI
e 95% CI
POD CP
X
POD CC
-
5
0
0.00 0.35
0.00, 0.43 0.18, 0.57
0 7
0.00 0.35
0.00, 0.43 0.00 0.18, 0.57 0.00
-0.43, 0.43 -0.28, 0.28
Instant Non- fat Dry Milk (25 g)
Salmonella Infantis ATCC 51741
0.54 (0.29, 0.91) 3.01 (1.31, 6.89)
20 Hours
20 7
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00 0.00
-0.43, 0.43
Analysi s Time Point
Presumptive
Confirmed
MPN a / Test Portion
Matrix/Test Portion
N b
f 95% CI g
Strain
dPOD CP
x c
d 95% CI
e 95% CI
POD CP
X
POD CC
-
5
0
0.00 0.40
0.00, 0.43 0.22, 0.61
0 8
0.00 0.40
0.00, 0.43 0.00 0.22, 0.61 0.00
-0.43, 0.43 -0.28, 0.28
Salmonella Tallahassee ATCC 12002
0.35 (0.16, 0.63) 3.01 (1.31, 6.89)
Black Pepper (25 g)
18 Hours
20 8
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00 0.00
-0.43, 0.43
Analysi s Time Point
Presumptive
Confirmed
MPN a / Test Portion
Matrix/Test Portion
N b
f 95% CI g
Strain
dPOD CP
x c
d 95% CI
e 95% CI
POD CP
X
POD CC
-
5
0
0.00 0.40
0.00, 0.43 0.22, 0.61
0 8
0.00 0.40
0.00, 0.43 0.00 0.22, 0.61 0.00
-0.43, 0.43 -0.28, 0.28
Cocoa Powder (25 g)
Salmonella Newport ATCC 6962
0.55 (0.29, 0.92) 2.29 (1.04, 5.02)
24 Hours
20 8
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00 0.00
-0.43, 0.43
Analysi s Time Point
Presumptive
Confirmed
MPN a / Test Portion
Matrix/Test Portion Pasteurized Liquid Whole Egg (100 g)
N b
f 95% CI g
Strain
dPOD CP
x c AOAC Research Institute Expert Review Panel Use Only POD CP d 95% CI X POD CC e 95% CI 5 0 0.00 0.00, 0.43 0.34, 0.74 0 0.00 0.55 0.00, 0.43 0.00 0.34, 0.74 0.00 0.68 (0.39, 1.12) 2.62 (1.20, 5.74) 20 11 0.55 11 5 5 1.00 0.57, 1.00 5 1.00 0.57, 1.00 0.00 -
-0.43, 0.43 -0.28, 0.28
Salmonella Enteriditis ATCC 13076
18 Hours
-0.43, 0.43
a MPN = Most Probable Number is calculated using the LCF MPN calculator provided by AOAC RI, with 95% confidence interval b N = Number of test portions c x = Number of positive test portions d POD CP = Candidate method presumptive positive outcomes divided by the total number of trials e POD CC = Candidate method confirmed positive outcomes divided by the total number of trials
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