AOAC ERP MICRO AUGUST 2018

OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016

Table 21: 3M ™ MDA 2 Salmonella Assay, Presumptive vs. Confirmed – POD Results

Analysi s Time Point

Presumptive

Confirmed

MPN a / Test Portion

Matrix/Tes t Portion

N b

f 95% CI g

Strain

dPOD CP

x c

d 95% CI

e 95% CI

POD CP

X

POD CC

-

5

0

0.00

0.00, 0.43 0.39, 0.78

0

0.00 0.55

0.00, 0.43 0.00 0.34, 0.74 0.05

-0.43, 0.43 -0.24, 0.33

Creamy Peanut Butter (25 g)

Salmonella Muenchen ATCC BAA 1594

0.55 (0.29, 0.93) 3.01 (1.31, 6.89)

18 Hours

20 12 0.60

11

5

5

1.00

0.57, 1.00

5

1.00

0.57, 1.00 0.00

-0.43, 0.43

Analysi s Time Point

Presumptive

Confirmed

MPN a / Test Portion

Matrix/Tes t Portion

N b

f 95% CI g

Strain

dPOD CP

x c

d 95% CI

e 95% CI

POD CP

X

POD CC

-

5

0

0.00 0.25

0.00, 0.43 0.11, 0.47

0 5

0.00 0.25

0.00, 0.43 0.00 0.11, 0.47 0.00

-0.43, 0.43 -0.26, 0.26

Creamy Peanut Butter (375 g)

Salmonella Muenchen ATCC BAA 1594

0.55 (0.29, 0.93) 3.01 (1.31, 6.89)

18 Hours

20 5

5

5

1.00

0.57, 1.00

5

1.00

0.57, 1.00 0.00

-0.43, 0.43

Analysi s Time Point

Presumptive

Confirmed

MPN a / Test Portion

Matrix/Tes t Portion

N b

f 95% CI g

Strain

dPOD CP

x c

d 95% CI

e 95% CI

POD CP

X

POD CC

-

5

0

0.00

0.00, 0.43 0.34, 0.74

0

0.00 0.55

0.00, 0.43 0.00 0.34, 0.74 0.00

-0.43, 0.43 -0.28, 0.28

Dry Dog Food (25 g)

Salmonella Typhimurium ATCC 14028

0.76 (0.44, 1.26) 4.38 (1.72, 11.15)

18 Hours

20 11 0.55

11

5

5

1.00

0.57, 1.00

5

1.00

0.57, 1.00 0.00

-0.43, 0.43

Analysi s Time Point

Presumptive

Confirmed

MPN a / Test Portion

Matrix/Tes t Portion

N b

f 95% CI g

Strain

dPOD CP

x c AOAC Research Institute Expert Review Panel Use Only POD CP d 95% CI X POD CC e 95% CI 5 0 0.00 0.35 0.00, 0.43 0.18, 0.57 0 7 0.00 0.35 0.00, 0.43 0.00 0.18, 0.57 0.00 0.76 (0.44, 1.26) 4.38 (1.72, 11.15) 20 7 5 5 1.00 0.57, 1.00 5 1.00 0.57, 1.00 0.00 -

-0.43, 0.43 -0.28, 0.28

Dry Dog Food (375 g)

Salmonella Typhimurium ATCC 14028

18 Hours

-0.43, 0.43

a MPN = Most Probable Number is calculated using the LCF MPN calculator provided by AOAC RI, with 95% confidence interval b N = Number of test portions c x = Number of positive test portions d POD CP = Candidate method presumptive positive outcomes divided by the total number of trials e POD CC = Candidate method confirmed positive outcomes divided by the total number of trials