AOAC ERP MICRO AUGUST 2018
OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016
Table 23: 3M ™ MDA 2 Salmonella Assay, Presumptive vs. Confirmed – POD Results
Analysi s Time Point
Presumptive x c POD CP
Confirmed
CFU a / Test Area
N b
f 95% CI g
Matrix
Strain
dPOD CP
d 95% CI
e 95% CI
X
POD CC
Salmonella Dublin STs 27 & Proteus vulgaris ATCC 6380
-
5
0 0.00
0.00, 0.43 0.39, 0.78
0
0.00
0.00, 0.43 0.39, 0.78
0.00 0.00
-0.43, 0.43 -0.28, 0.28
32 & 510
20 12 0.60
12 0.60
Sealed Concrete
18 Hours
400 & 3700
5
5 1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
Analysi s Time Point
Presumptive x c POD CP
Confirmed
CFU a / Test Area
N b
f 95% CI g
Matrix
Strain
dPOD CP
d 95% CI
e 95% CI
X
POD CC
-
5
0 0.00
0.00, 0.43 0.58, 0.92 0.57, 1.00
0
0.00
0.00, 0.43 0.58, 0.92 0.57, 1.00
0.00 0.00 0.00
-0.43, 0.43 -0.25, 0.25 -0.43, 0.43
Stainless Steel
Salmonella Kahla ATCC 17980
18 Hours
57
20 16 0.80
16 0.80
510
5
5 1.00
5
1.00
Analysi s Time Point
Presumptive x c POD CP
Confirmed
CFU a / Test Area
N b
f 95% CI g
Matrix
Strain
dPOD CP
d 95% CI
e 95% CI
X
POD CC
-
5
0 0.00
0.00, 0.43 0.15, 0.52 0.57, 1.00
0 6 5
0.00 0.30 1.00
0.00, 0.43 0.15, 0.52 0.57, 1.00
0.00 0.00 0.00
-0.43, 0.43 -0.27, 0.27 -0.43, 0.43
Sealed ceramic Tile
Salmonella Anatum ATCC 9270
18 Hours
40
20 6 0.30
470
5
5 1.00
a CFU/Test Area = Results of the CFU/Test area were determined by plating the inoculum for each matrix in triplicate b N = Number of test portions c x = Number of positive test portions d POD CP = Candidate method presumptive positive outcomes divided by the total number of trials e POD CC = Candidate method confirmed positive outcomes divided by the total number of trials f dPOD CP = Difference between the candidate method presumptive result and candidate method confirmed result POD values g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level
AOAC Research Institute Expert Review Panel Use Only
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