AOAC ERP MICRO AUGUST 2018
B ird et al .: J ournal of AOAC I nternational V ol . 100, N o . 1, 2017 89
Table 2016.07D. Comparative results for the detection of Listeria in 25 g raw chicken breast fillet test portions by the 3M MDA 2– Listeria method versus USDA/FSIS MLG 8.09 in the collaborative study Uninoculated control
CP a
CC b
C c
R d
C vs R
Matrix/ inoculation level Raw chicken breast fillet
N e
x f
Statistic
POD CP
POD CC
POD C
POD R
dLPOD C vs R
dLPOD CP vs CC
Lab
N x
N x
N x
1 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 2 12 1 0.08 12 1 0.08 12 1 0.08 12 0 0.00 3 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 4 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 5 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 6 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 7 12 1 0.08 12 0 0.00 12 0 0.00 12 0 0.00 8 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 9 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 12 2 0.17 12 0 0.00 12 0 0.00 11 h NA NA NA NA NA NA NA NA NA NA NA NA 12 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 13 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 10 g
0.00 0.08 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
0.00 0.00 0.00 0.00 0.00 0.00 0.08 0.00 0.00
–0.17
NA
NA
0.00 0.00
0.00 0.00
POD CC
POD C
POD R
dLPOD C vs R
dLPOD CP vs CC
Labs
POD CP
N x
N x
N x
N x
Estimate
All 132 2 0.02 132 1 0.01 132 1 0.01 132 0 0.00
0.01
0.01
LCL i UCL j
0.00 0.06 0.12 0.11 0.16 0.00 0.00 0.05 0.12 0.11 0.14
0.00 0.04 0.09 0.08 0.16 0.00 0.00 0.04 0.09 0.08 0.10
0.00 0.04 0.09 0.08 0.16 0.00 0.00 0.04 0.09 0.08 0.10
0.00 0.03 0.00 0.00 0.16 0.00 0.00 0.16 0.00 0.00 0.23
–0.02
–0.03
0.04
0.05
s r
LCL UCL
k
s L
LCL UCL
s R
UCL LCL
l
0.5190
0.4338
0.4338
1.0000
P T
Low inoculum level
Raw chicken breast fillet
1 12 7 0.58 12 7 0.58 12 7 0.58 12 7 0.58 2 12 9 0.75 12 9 0.75 12 9 0.75 12 7 0.58 3 12 10 0.83 12 10 0.83 12 10 0.83 12 5 0.42 4 12 9 0.75 12 9 0.75 12 9 0.75 12 4 0.33 5 12 8 0.66 12 8 0.66 12 8 0.66 12 5 0.42 6 12 10 0.83 12 9 0.75 12 9 0.75 12 6 0.50 7 12 8 0.66 12 6 0.50 12 6 0.50 12 6 0.50 8 12 7 0.58 12 7 0.58 12 7 0.58 12 7 0.58 9 12 8 0.66 12 9 0.75 12 8 0.66 12 7 0.58 12 6 0.50 12 8 0.66 12 6 0.50 12 5 0.42 11 h NA NA NA NA NA NA NA NA NA NA NA NA 12 12 7 0.58 12 7 0.58 12 7 0.58 12 4 0.42 13 12 5 0.42 12 5 0.42 12 5 0.42 12 6 0.50 ERP Use Only 0.00 0.17 0.41 0.42 0.22 0.15 0.00 0.00 0.08 0.08 10 g NA 0.16 –0.08
0.00 0.00 0.00 0.00 0.00 0.08 0.16 0.00
–0.08 –0.16
NA
MPN/test portion
0.00 0.00
POD CC
POD C
POD R
dLPOD C vs R
dLPOD CP vs CC
Labs
POD CP
N x
N x
N x
N x
Estimate 0.66
All 132 88 0.67 132 86 0.65 132 85 0.64 132 64 0.48
0.16 0.04 0.28
0.02
LCL
0.51
0.58 0.75 0.48 0.42 0.52
0.57 0.73 0.48 0.43 0.52
0.56 0.73 0.48 0.43 0.52
0.40 0.57 0.51 0.46 0.52
–0.10
UCL 0.83
0.13
s r
LCL UCL
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