AOAC ERP MICRO AUGUST 2018
OMAMAN-29 D/ PTM Validation Report 111501 OMA ERP - June 2016 ERP Use Only
Table 12: 3M ™ MDA 2 - Listeria Assay, Presumptive vs. Confirmed – POD Results (INTERNAL STUDY)
Analysis Time Point
Presumptive
Confirmed
MPN a / Test Portion
N b
95% CI g
dPOD CP f
Matrix
Strain
x c
d
POD CC e
POD CP
95% CI 0.00, 0.43 0.08, 0.42 0.57, 1.00 95% CI 0.00, 0.43 0.38, 0.96 0.12, 0.77 95% CI 0.00, 0.43 0.15, 0.52 0.57, 1.00 95% CI 0.00, 0.43 0.30, 0.70
X
95% CI 0.00, 0.43 0.26, 0.66 0.57, 1.00 95% CI 0.00, 0.43 0.38, 0.96 0.12, 0.77 95% CI 0.00, 0.43 0.15, 0.52 0.57, 1.00 95% CI 0.00, 0.43 0.30, 0.70
-
5
0 4 5
0.00 0.20 1.00
0 9 5
0.00 0.45 1.00
0.00 -0.25 0.00
-0.43, 0.43 -0.49, 0.05 -0.43, 0.43
Raw Chicken Leg Pieces h (25 g)
28 Hours
L. ivanovii Ad 1291
0.31 (0.14, 0.57)
20
0.5
5
Analysis Time Point
Presumptive
Confirmed
MPN a / Test Portion
N b
95% CI g
dPOD CP f
Matrix
Strain
x c
d
POD CC e
POD CP
X
-
5
0 4 2
0.00 0.80 0.40
0 4 2
0.00 0.80 0.40
0.00 0.00 0.00
-0.43, 0.43 -0.25, 0.25 -0.46, 0.46
Raw Chicken Fillet i (25 g)
28 Hours
L. ivanovii Ad 1291
0.3 (0.1, 0.5)
20
0.5
5
Analysis Time Point
Presumptive
Confirmed
MPN a / Test Portion
N b
95% CI g
dPOD CP f
Matrix
Strain
x c
d
POD CC e
POD CP
X
-
5
0 6 3
0.00 0.30 1.00
0 6 3
0.00 0.30 1.00
0.00 0.00 0.00
-0.43, 0.43 -0.27, 0.31 -0.43, 0.43
Bagged Raw Spinach h (25 g)
24 Hours
L. seeligeri Ad 1754
0.26 (0.10, 0.47)
20
1.7
5
Analysis Time Point
Presumptive
Confirmed
MPN a / Test Portion
N b
95% CI g
dPOD CP f
Matrix
Strain
x c
d
POD CC e
POD CP
X
-
5
0
0.00 0.50
0
0.00 0.50
0.00 0.20
-0.43, 0.43 -0.26, 0.62
Cold Smoked Salmon h (25 g)
0.27 (0.1, 0.49)
20
10
10
24 Hours
L. innocua Ad 1674
1.7
5
5
1.00
0.57, 1.00
5
1.00
0.57, 1.00
0.00
-0.43, 0.43
a MPN = Most Probable Number is calculated using the LCF MPN calculator provided by AOAC RI, with 95% confidence interval b N = Number of test portions c x = Number of positive test portions d POD CP = Candidate method presumptive positive outcomes divided by the total number of trials e POD CC = Candidate method confirmed positive outcomes divided by the total number of trials f dPOD CP = Difference between the candidate method presumptive result and candidate method confirmed result POD values g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level
AOAC Research Institute Expert Review anel Use Only
h Tested using Demi Fraser brand (with FAC) brand X. I Tested using Demi Fraser brand (with FAC) brand Z.
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