AOAC ERP MICRO AUGUST 2018

OMAMAN-44 A: Collaborative Study Manuscript Expert Review Panel Use Only August 2018

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Among the most notable foodborne pathogens and spoilage organisms are Escherichia, Salmonella,

Enterobacter, Klebsiella, Citrobacter, Cronobacter, Shigella and Yersinia . Methods for the detection and 99

enumeration of Enterobacteriaceae have changed very little since they were first introduced and many 100

still rely on the growth of the bacterium in selective media along with the use of carbohydrates as an 101

energy source (7). Because Enterobacteriaceae are used so frequently by the food industry, there are 102

needs for simple, low cost, ready-to-use methods for testing. Peel Plate EB is a simple method to detect 103

and quantify Enterobacteriaceae in foods that is being studied and validated in this work. 104

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Materials and Methods

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Test Kit Information

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( a )

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Kit Name .—Peel Plate EB.

( b ) Catalog Number .—PP-EB-100K, 100 Peel Plate EB tests; PP-EB-1000K, 1000 Peel Plate EB Tests

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( c ) Ordering Information .—Charm Sciences, Inc., 659 Andover St., Lawrence, MA 01843 USA Tel.

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978-687-9200, Fax 978-687-9216 email: info@charm.com, www.charm.com.

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Test Kit Components

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( a ) Two foil bags containing 50 Peel Plate EB each with blue indicator desiccants . ERP Use Only Additional Supplies and Reagents

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( a ) Butterfield’s Phosphate Buffered Dilution Water (BPBDW).—Buffer KH 2 PO 4

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(34 g to 500 mL

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