AOAC ERP MICRO AUGUST 2018

OMAMAN-44 A: Collaborative Study Manuscript Expert Review Panel Use Only August 2018

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sponge to buffer and stomaching for 1–2 min.

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Chicken Carcasses:

( a ) The sampling protocol followed FSIS Directives for chicken carcass (9, 10). 400mL BPW or n-

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BPW were added to a bag with a chicken carcass, bag was sealed and shaken for 1 minute (11). 100 mL 173

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of rinsate was collected for testing.

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Method Procedure

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( a ) Place Peel Plate onto a level surface. Apply pressure with fingers to the rear rectangular

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platform to keep plate flat.

( b ) Lift cover vertically upwards completely exposing the dried media culture disc. Leave cover

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adhered to back of plate.

( c ) While holding cover up, keep plate flat on surface, vertically dispense 1.0 mL of sample or

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sample dilution to the center of exposed Peel Plate disc. Expel pipet contents rapidly with even force 183

and within 2 to 3 s. Sample will self-wick to the edges of the disc. It is acceptable to lift and rotate plate 184

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to swirl sample to edges when sample conditions interfere with wicking.

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( 1 ) In the case of cereal, 5 plates, should be rehydrated per sample. Alternatively, 5 mL

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homogenized sample are added to one high volume plate.

( d ) Re-apply the adhesive cover without wrinkling. Press cover around edges of plate to ensure ERP Use Only ( e ) Incubate plates with adhesive cover down, clear side up. ( 1 ) Incubate at 37 ± 1°C for 24 up to 48 h.

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proper seal.

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( 2 ) Plates can stack by aligning the 3 feet. Stacking up to 20 will not affect plate heat transfer.

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