AOAC Final Action Methods in 2017

where 40 = dietary conversion factor (μg/hg to IU/hg). K. Data Handling Report results as μg/hg to one decimal place or as IU/hg to zero decimal places. References: J. AOAC Int . 99 , 1321(2016) DOI: 10.5740/jaoacint.16-0160 (First Action) J. AOAC Int . 101 , 256(2018) DOI: 10.5740/jaoacint.17-0149 (Final Action) AOAC SMPR 2011.004 J. AOAC Int . 95 , 292(2012) DOI: 10.5740/jaoac.int.11-0440 * Method adopted by AOAC as an Official Method of Analysis SM and by the International Organization for Standardization as ISO 20636:2018. Posted: February 2018, July 2018

SILIS

PA

SILIS

100

=

×

×

×

alqt

NLD

Dconcn

Result D

PA

L

S

1000

SILD

vol

where Result D = vitamin D 2 or vitamin D 3 concentration in the sample (μg/dL); PA NLD = peak area of vitamin D 2 or vitamin D 3 in the sample; PA SILD = peak area of d6 -vitamin D 2 or d6 -vitamin D 3 in the sample; SILIS Dconcn = concentration of d6 -vitamin D 2 or d6 - vitamin D 3 in the SILIS (ng/mL); L = slope of the calibration curve; SILIS alqt = volume of the SILIS aliquot spiked into the sample (0.5 mL); S vol = volume of the sample (mL); 1000 = concentration conversion factor (ng/mL to μg/mL); and 100 = concentration conversion factor (μg/mL to μg/dL). (o)  The concentration of vitamin D 2 or vitamin D 3 as IU/hg in the sample is calculated as Result IU hg Result g/hg 40 ( ) ( ) = m ´

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