AOAC Final Action Methods in 2018

Table 2011.14A. Interlaboratory study results for calcium (Ca)

Original validation (2011)

Matrix

Mean, mg/kg Rec., % s r a

RSD r

b , % s

R c , mg/kg RSD R

d , % HorRat

No. of labs e Outlier labs f

Chocolate milk powder Dietetic milk powder 2

9732.17 5678.58 5983.54

99 98

152.10

1.56 1.34 3.52 3.26 0.95

264.03 184.11 251.67

2.71 3.24 4.21 6.88 3.51 4.65 5.82 8.19 3.30 5.49 8.20 3.88 7.20 27.8 5.30 10.3

0.68 0.74 0.97 1.06 0.53 1.06 1.05 1.46 0.79 1.14 0.87 1.09 1.82 1.77 1.12 1.69 1.17 1.58 1.35 1.32 1.33 1.13 1.29 1.32 2.05 0.53 1.19 1.3 4.5 0.7

9 9 9 9 7

NS g

75.93

NS NS NS

Infant cereal

100 100 100

210.58

Peanut butter NIST SRM 2387 Wheat gluten NIST RM 8418

409.90 (411) h 367.56 (369) h

13.37

28.20 12.90

3.51

2, 4C

Matrix extension (2017)

Mean, mg/100 g i

SRM 1849a Whole milk

530 (525)

101 ND j

12.7 2.89

2.4

24.7 6.77 8.61 23.7 37.8 1.62 19.2 2.49 15.4

12 11 10

0 0 1 2 0 4 0 0 1 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 0

116 105 709 689 19.8 494 34.6 55.5 14.5 7.44 34.4 49.5 38.2 68.9 63.2 45.7 69.5 36.7 74.4 48.9 30.3 65.9 99 58

2.48 3.28 1.45 4.86 2.10 1.69 4.29

Whole milk powder

ND 3.45 ND 10.3 ND 33.5 ND 0.42 ND 8.37 ND 1.48

Whey powder

9

Whey protein concentrate

11

Butter

7

Cheese

11 12 11 12 12 12 12 12 11 11 12 10 11 12 12 12 12 12 12

Adult powder, low fat Adult RTF, high fat

ND ND ND

5.1 0.3 7.2

9.3 2.2 7.3

Adult RTF, high fat placebo Adult RTF, high protein Adult RTF, high protein placebo Child milk powder placebo

0.8

10.2

ND 0.167 ND 0.91 ND 2.33 ND 0.98

2.25 2.64

1.102

14.80

2.57 5.27 2.93

7.47

Child milk powder

4.7

10.60

Child elemental powder Infant elemental powder

2.56

7.7

ND

2.7

3.9

6.5

9.50

Infant elemental powder placebo

ND 1.34

2.13

5.18

8.2

Infant PH powder milk Infant PH powder soy Infant powder FOS-GOS

ND

0.4

0.9 3.2

3.8

8.40

ND 2.22 ND 0.774 ND 2.18 ND 2.84 ND 4.14 ND 0.63 ND 1.57

5.52 2.72 4.59 5.81 6.32 1.08 4.73

7.9

2.11 3.76 3.81 8.46 2.08 2.38

7.42 7.92 7.80 12.9 3.56 7.17

Infant powder milk Infant powder soy

Infant RTF milk

Infant RTF placebo

Toddler powder

a  s r = Standard deviation for repeatability (within laboratory). b  RSD r = Relative standard deviation for repeatability. c  s R = Standard deviation for reproducibility (among laboratories). d  RSD R = Relative standard deviation for reproducibility. e  Reporting laboratories minus outliers. f  C = Cochran outlier; SG = Single Grubbs outlier; DG = Double Grubbs outlier. g  NS = Not significant. h  Value in parentheses = Certified or reference values. i  Results expressed as mg/100 g for ready-to-feed liquids as-is or reconstituted powders (25 g in 200 g water) or liquid concentrates (1:1 dilution by weight). j  ND = Not determined.

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