AOAC Final Action Methods in 2019

μg; M S = sample weight, in g; A Lyc = peak area of all- trans lycopene in the sample chromatogram, in arbitrary units; A XcisLyc = peak area of cis isomers of lycopene in the sample chromatogram, in arbitrary units; A A = peak area of apocarotenal in the sample chromatogram, in arbitrary units; I Lyc = y -intercept of the calibration curve for all- trans lycopene; RF Lyc = slope of the calibration curve for all- trans lycopene. References: J. AOAC Int . 100 , 758(2017) DOI: 10.5740/jaoacint.16-0386 (First Action) J. AOAC Int . (future issue) DOI: 10.1093/jaocint/qsz017 (Final Action β-carotene and lycopene only) AOAC SMPR 2014.014 (Carotenoids) J. AOAC Int . 98 , 1077(2015)

in the sample chromatogram, in arbitrary units; I BC = y -intercept of the calibration curve for all- trans β-carotene; RF BC = slope of the calibration curve for all- trans β-carotene. BC cis = (M A /M S ) × ([([A 15cisBC × 1.4] + [A 13cisBC × 1.2] + A 9cisBC + A XcisBC )/A A ] – I BC ) × (100/RF BC ) where BC cis = cis isomers of β-carotene in the sample (μg/100 g); M A = mass of apocarotenal added to the test sample, in μg; M S = sample weight, in g; A 15cisBC = peak area of 15- cis β-carotene in the sample chromatogram, in arbitrary units; A 13cisBC = peak area of 13- cis β-carotene in the sample chromatogram, in arbitrary units; A 9cisBC = peak area of 9- cis β-carotene in the sample chromatogram, in arbitrary units; A XcisBC = peak area of unidentified cis isomers of β-carotene in the sample chromatogram, in arbitrary units; A A = peak area of apocarotenal in the sample chromatogram, in arbitrary units; I BC = y -intercept of the calibration curve for all- trans β-carotene; RF BC = slope of the calibration curve for all- trans β-carotene. BC Total = BC trans + BC cis ( l ) Calculate the content of total lycopene on an as-is basis in the test samples. For peak identification, the first cis lycopene peak has a relative retention time of approximately 0.87 compared to all- trans lycopene, and any peaks between the first cis peak and all- trans lycopene are also cis peaks. See Figure 2016.13F . Lyc Total = (M A /M S ) × [(A Lyc + A XcisLyc )/A A ] – I Lyc ) × (100/RF Lyc ) where Lyc Total = total trans and cis isomers of lycopene in the sample (μg/100 g); M A = mass of apocarotenal added to the test sample, in

DOI: 10.5740/jaoac.int.SMPR2014.014 AOAC SMPR 2017.006 (β-carotene) J. AOAC Int . 100 , 1217(2017) DOI: 10.5740/jaoacint.SMPR2017.006 AOAC SMPR 2017.007 (Lutein) J. AOAC Int . 100 , 1219(2017) DOI: 10.5740/jaoacint.SMPR2017.007 AOAC SMPR 2017.008 (Lycopene) J. AOAC Int . 100 , 1221(2017) DOI: 10.5740/jaoacint.SMPR2017.008

Posted: June 2017 (First Action); November 2018 (Revised First Action to include lycopene); October 2019 (Final Action for β -carotene and lycopene only); April 2020 (Final Action publication)

© 2020 AOAC INTERNATIONAL