AOAC 2019 First Action Methods

Table 2019.09C. Gradient program

Time (min)

Flow (mL/min)

Mobile phase A (%)

Mobile phase B (%)

Curve

0.000 3.500 4.400 6.389 7.500

0.600 0.600 0.600 0.600 0.600 0.300 0.300 0.300 0.600 0.600 0.600 0.600 0.600 0.600

99.9 99.9 98.0 94.0 87.9 82.0 81.0 74.0 70.0 40.4 10.0 10.0 99.9 99.9

0.1 0.1 2.0 6.0

5 5 5 5 5 5 5 5 5 5 5 5 5 5

12.1 18.0 19.0 26.0 30.0 59.6 90.0 90.0

10.130 10.660 13.500 15.000 15.500 15.700 16.500 17.000 20.000

0.1 0.1