AOAC 2019 First Action Methods

Additional Items

( a ) Incubators .― Capable of maintaining 35 ± 1ºC or 37 ± 1ºC. ( b ) Adjustable, variable volume pipets .―Capable of sampling and delivering 10 –20 µL. ( c ) Adjustable, variable volume pipets (single or multichannel) .― Capable of sampling and delivering 0.5– 10 µL. ( d ) Compatible sterile, filter pipet tips .―20 µL. ( e ) Refrigerator .―Capable of maintaining 2 – 8°C. ( f ) Vortex-Genie Pulse .―Scientific Industries, SKU: SI -P236. C. General Instructions ( a ) The GENE-UP® Listeria spp. 2 (LIS 2) kit is for professional use only with the GENE-UP® Lysis kit. ( b ) Comply with Good Laboratory Practices. ( c ) Do not use reagents after the expiration date indicated on the label. ( d ) Visually inspect vials before testing. Do not use vials with evidence of damage, leakage, or deterioration. ( e ) Do not mix reagents (or disposables) from different lots. ( f ) Powder-free latex or nitrile gloves are recommended for all PCR steps. ( g ) The equipment and accessories should be regularly cleaned and decontaminated. ( h ) Never remove the caps from the lysis tubes. ( i ) Do not try to remove the strip tube caps once they have been sealed to the PCR strip tubes. ( j ) Spills should be wiped up thoroughly after treatment with bleach or a nucleic acid degradation solution. See the GENE-UP® user manual for information on cleaning spills on or in the instrument. Do not autoclave solutions containing bleach. D. Sample Enrichment [Allow the enrichment broths to reach 15–25°C before use. For select matrices, pre-warm LPT broth to 35 ± 1°C prior to use. Frozen samples should be thawed before analysis. Use a blender bag containing filter.] ( a ) Deli ham, deli turkey, beef hot dogs, breaded chicken nuggets, fresh bagged spinach, fresh bagged lettuce, cooked shrimp, smoked salmon, vanilla ice cream, Mexican soft cheese, pork rillettes, raw milk,