AOAC 2019 First Action Methods

Table 2019.11A . Summary of results for the detection of Listeria monocytogenes in full-cream goat milk cottage cheese (8.4% Fat) Method a GENE-UP ® Listeria monocytogenes 2 (LMO 2)

Extraction Method Inoculation Level

Non-inoculated

Low

High

Candidate Presumptive Positive/ Total # of Samples Analyzed Candidate Presumptive LPOD (CP)

4/120

90/120

120/120

0.03 (0.01, 0.08)

0.75 (0.59, 0.91)

1.00 (0.97, 1.00)

s r s L s R

0.16 (0.14, 0.19) 0.08 (0.04, 0.15) 0.18 (0.16, 0.22)

0.34 (0.30, 0.39) 0.28 (0.18, 0.46) 0.44 (0.38, 0.53)

0.00 (0.00, 0.17) 0.00 (0.00, 0.17) 0.00 (0.00, 0.24)

b

c

d

P Value e

0.0017

0.0000

1.0000

Candidate Confirmed Positive/ Total # of Samples Analyzed Candidate Confirmed LPOD (CC)

2/120

90/120

120/120

0.02 (0.00, 0.06)

0.75 (0.59, 0.91)

1.00 (0.97, 1.00)

s r s L s R

0.12 (0.11, 0.17) 0.05 (0.02, 0.09) 0.13 (0.12, 0.16)

0.34 (0.30, 0.39) 0.28 (0.18, 0.46) 0.44 (0.38, 0.53)

0.00 (0.00, 0.17) 0.00 (0.00, 0.17) 0.00 (0.00, 0.24)

P Value

0.0123

0.0000

1.0000

Candidate Confirmed Positive/ Total # of Samples Analyzed Candidate Presumptive Positive that Confirmed LPOD (C)

2/120

90/120

120/120

0.02 (0.00, 0.06)

0.75 (0.59, 0.91)

1.00 (0.97, 1.00)

s r s L s R

0.12 (0.11, 0.17) 0.05 (0.02, 0.09) 0.13 (0.12, 0.16)

0.34 (0.30, 0.39) 0.28 (0.18, 0.46) 0.44 (0.38, 0.53)

0.00 (0.00, 0.17) 0.00 (0.00, 0.17) 0.00 (0.00, 0.24)

P Value

0.0123

0.0000

1.0000

Positive Reference Samples/ Total # of Samples Analyzed

4/120

99/120

120/120

Reference LPOD

0.03 (0.01, 0.08) 0.17 (0.15, 0.20) 0.06 (0.02, 0.13) 0.18 (0.16, 0.22)

0.83 (0.66, 0.99) 0.26 (0.23, 0.30) 0.29 (0.20, 0.47) 0.39 (0.33, 0.53)

1.00 (0.97, 1.00) 0.00 (0.00, 0.17) 0.00 (0.00, 0.17) 0.00 (0.00, 0.24)

s r s L s R