AOAC Gluten Qualitative Validation Guidance-FINAL (July 2023)

Annex C Matrix Categories

NOT FERMENTED, HYDROLYZED OR FRACTIONATED

*

PROCESSED / BAKED / CURED / SMOKED / MARINATED

PRESSURE/HEAT-UHT/ PASTEURIZATION/ EXTRUSION

DEHYDRATED / DRIED / DRY CURED

RAW/ MINIMALLY PROCESSED RAW/ MINIMALLY PROCESSED

FOOD CATEGORY

FERMENTED

HYDROLYZED

FRACTIONATED

FRIED

FERMENTED

HYDROLYZED

FRACTIONATED

Binders, stabilizers, emulsifiers

Xanthan gum, Guar gum

Carrageenan

PROCESSED

Caramel, pralines, marzipan, nougats,

Pastilles, Lozenges, jelly beans, toffees, licorice, Chewing gum, Mints, Icing or Frosting (non chocolate), sauces used for toppings, non chocolate

Candy

(butterscotch, marshmallow)

RAW/ MINIMALLY PROCESSED

PROCESSED, BAKED

FRIED

PRESSURE/EXTRUSION

DEHYDRATED

FERMENTED

HYDROLYZED

FRACTIONATED

Soy Milk, Oat milk, Rice milk, Teff milk (if hydrolyzed as part of processing); Modified food starch.

Bread, cakes, cookies, tortillas, fresh pasta, bakery products, confectionaries, crackers, bagels, muffins, grain-based protein bars

Sourdough, Malt, Malt extract, Sprouted flours, Soy, oat or rice milk yogurts, natto, tempeh, soy sauce, miso

Whole or milled Sorghum, soybeans, corn, millet, teff, rice, fonio, oats; baking mixes

Breaders / Batters for fish sticks and chicken nuggets, tortilla chips, donuts

Unmodified Wheat starch, soy protein isolate, tofu, Maltodextrin, Soy Lecithin

Breakfast cereals, Puffs /pellets

Bread crumbs, Dried Pasta

Cereal Grains

RAW/ MINIMALLY PROCESSED

Chemicals and Preservatives

Need to be validated per matrix RAW/ MINIMALLY PROCESSED

PROCESSED

Cocoa powder, cocoa sugar mixes, Baking chocolate, Chocolate chips, chocolate bars, chocolate syrup, cocoa butter, chocolate hazelnut spread, chocolate frosting, enrobing chocolate, and similar carob-based products

Chocolate and Cocoa

cocoa nibs, cocoa mass,

Rinsates, CIP rinse - validate per cleaning solution RAW/ MINIMALLY PROCESSED Caramel color, caramel flavour, liquid smoke, Beverage flavorings for water, coffee

Cleaning Solutions and Rinses

DRIED

Spirulina, Beetroot powder, Pitaya powder, acai berry powder, matcha green tea powder, turmeric, saffron, Annatto extract

Colors, Flavors and Fragrances (other than those prepared as extracts)

FERMENTED

Validate per culture strain

Cultured Materials

RAW/ MINIMALLY PROCESSED

PASTEURIZED/ HEATED/ UHT

PROCESSED

FERMENTED

Raw or pasteurized Greek yogurt, Skyr, Semi-hard cheeses (eg Comte, Beaufort), Blue cheese (Roquefort), Soft cheese (eg Brie, Munster)

Whipped Cream, Cream Cheese, Custards, Puddings

Dairy Products (High Fat)

Ice cream, Canned creams

Raw butter, Raw cream