AOAC Gluten Qualitative Validation Guidance-FINAL (July 2023)

Gluten Public Comments (Responses)

should be one of the more challenging matrices (most highly processed). Differences between gluten sources will thus be tested in the first matrix, differences between matrices with wheat (or highest CDC cereal) in all other tests. 4.1.3 Table 1 and 2 Table 1 and 2 are Confusing. Consider reformatting like this:

Per WG discussion, the tables were reformatted to match the example above, addressing all three prior comments. ------------------------------------------------------------------------------------------------------------------------------------------ 4.1.6 Page 7 “Biological replicates” This is still confusing to me. “Test ten biological replicates for each factorial pattern” ?? What is a “factorial pattern?” I think you mean “factorial combination” or maybe “factorial treatment combination” I would replace with the following: “Analyze ten replicate test portions from the same test material for each factorial treatment combination.” 4.1.6 Page 7 “POD should be calculated for each factorial pattern.” I suppose you could do it that way – better to just say “Experimental results should be calculated as described in Annex D.” Section 4.1.6, second paragraph, final sentence was changed from “Test ten biological replicates for each factorial pattern” to “test ten (10) test portions for each factorial treatment combination”. Third and fourth paragraphs, which read “POD should be calculated for each factorial pattern, and analyzed as described in Annex D. Data should be recorded in a table that describes each individual parameter variation, the individual results of each of the ten replicates for each factorial pattern, and the POD for each factorial pattern. The results of the statistical analysis should be reported in a separate table.” were replaced with a single sentence that reads “Data should be reported and analyzed as described in Annex D.” ------------------------------------------------------------------------------------------------------------------------------------------