AOAC GFA Stakeholder Program Meeting Book (March 15, 2023)

LOD and LOQ

Basic approach was used LOD = mean (blank matrix) + 3 x standard deviation (blank). LOQ= mean (blank matrix) + 10 x standard deviation (blank).

Claimed

Muffin

Calibrator

Spice Mix

Hummus

LOD b LOQ b 0.36 c 0.875 c 0.06 ± 0.04 0.18 ± 0.12 LOD ± SD LOQ ± SD

0.19 ± 0.24 0.63 ± 0.81 0.18 ± 0.10 0.56 ± 0.32 0.22 ± 0.15 0.76 ± 0.51 LOD ± SD LOQ ± SD

0.44 ± 0.61 1.50 ± 2.06 0.09 ± 0.07 0.30 ± 0.22 0.78 ± 1.05 2.94 ± 4.04 LOD ± SD LOQ ± SD

0.29 ± 0.31 0.96 ± 1.03 0.36 ± 0.43 1.08 ± 1.25 3.16 ± 4.88 9.55 ± 14.69 LOD ± SD LOQ ± SD

ELISA kit A ELISA kit B ELISA kit C

0.14 2.5

0.20 ± 0.18 0.64 ± 0.53 0.31 ± 0.37 1.12 ± 1.34

0.5

2.5

LOD, LOQ expressed as ppm sesame ± indicates variation among the three analysts

23