AOAC GFA Stakeholder Program Meeting Book (March 15, 2023)

Recovery and precision ELISA kit C

Analyst 3

Analyst 1

Analyst 2

0.10 ± 0.003 1.19 ± 0.21 1.81 ± 0.37 2.68 ± 0.18 Avg. OD ± SD e 0.23 ± 0.004 0.92 ± 0.03 1.44 ± 0.20 2.31 ± 0.20 0.13 ± 0.01 0.11 ± 0.02 0.32 ± 0.08 0.84 ± 0.56 0.08 ± 0.00 0.08 ± 0.03 0.08 ± 0.01 0.14 ± 0.02

0.08 ± 0.004 1.26 ± 0.10 1.74 ± 0.29 2.95 ± 0.09 Avg. OD ± SD e 0.18 ± 0.04 1.07 ± 0.16 1.46 ± 0.22 2.74 ± 0.01 0.21 ± 0.15 0.13 ± 0.06 0.04 ± 0.01 0.32 ± 0.26 0.12 ± 0.01 0.10 ± 0.03 0.10 ± 0.02 0.15 ± 0.01

0.10 ± 0.01 0.71 ± 0.06 1.14 ± 0.21 2.05 ± 0.03 Avg. OD ± SD e 0.13 ± 0.003 0.54 ± 0.07 1.00 ± 0.03 1.84 ± 0.06 0.09 ± 0.002 0.18 ± 0.15 0.13 ± 0.02 0.39 ± 0.29 0.09 ± 0.001 0.06 ± 0.01 0.13 ± 0.02 0.12 ± 0.01

RSD ppm f

RSD ppm f

Sample ID ppm

RSD ppm f

% Rec. g

% Rec. g

% Rec. g

Muffin b

M-0 d

0 5

23.2 28.1 i

19.6 25.8 i -12.7 i

22.5 29.5 i 15.0 i

M-5

7.5

465 281

7.1

392 258 -42

16.5 19.4

451 295

M-10 M-30

10 30

16.1

16.9

2.0 i

50

6.5

3.0

7

1.5

Spice Mix

S-0 d

0 5

19.9 25.8 i 11.8 i

15.2 24.9 4.8 i

17.2 26.5 i 25.7 i

S-5

13.0 13.3

398 258

9.8 2.5 3.1

303 249

2.9

343 265

S-10 S-30

10 30

11.9

86

16

7.9

0.2

39

Hummus c

S-0 d

0 5

(Day 1)

1.7 0.5 5.4

3.2 2.2 8.5

1.6 3.9

H-5

17.8

33

54.8 10.3 60.7

64 22 28

6.7

32 39 34

H-10 H-30

10 30

2.4

5

17.6 57.8

10.3

49.5

18

Hummus c

S-0 d

0 5

(Day 7)

1.8 1.8 2.8

0.7 2.0 1.7

2.9 3.0 5.2

H-5

15.6

35 18

4.5 8.4 3.0

15 20

17.3

58 30 17

H-10 H-30

10 30

9.7 5.4

3.4 7.4

9

6

RSD exceeding 30% were observed with multiple hummus day 1 samples for all analysts All other samples RSD <20% % recovery was very high up to 465% for muffin and 398% for spice mix Very low recovery (<10% for some) for hummus samples.

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