AOAC GFA Stakeholder Program Meeting Book (March 17, 2021)

Method Validation Guidance

X AOAC Appendix M: Validation Procedures for Quantitative Food Allergen ELISA Methods: Community Guidance and Best Practices

X AOAC Appendix D: Guidelines for Collaborative Study Procedures To Validate Characteristics of a Method of Analysis X AOAC Appendix N: ISPAM Guidelines for Validation of Qualitative Binary Chemistry Methods

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Appendix M

Data Analysis for Interlaboratory Studies

Allergen-Specific Information

Interlaboratory Validation

Antibody information

Number of Laboratories

Reference Materials

Cross-reactivity

Matrixes, Concentration Levels, and Replicates

Calibrators

Spiking Methods

Matrixes

LOQ, LOD, LLA Ruggedness and lot-to- lot variability

Acceptance Criteria

Food Matrixes

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