AOAC HMO-06 Reviewer Forms (July 25, 2023)

FOR EXPERT REVIEW PANEL USE ONLY NOT FOR DISTRIBUTION

Expert Review Panel for Infant Formula and Adult Nutrition Evaluation of Method HMO-06

Title: AOAC Official Method 20xx.xx Analysis of Eight Human Milk Oligosaccharides (HMOs) in Infant Formula and Premix Samples by Labelling with Benzocaine and Analysis by HPLC-UV First Action 20xx Author: Dora Molnar-Gabor, Marton Lengyel, Teddy Krongaard Reviewer Name: Reviewer 1 Summary of Method: This method uses a labeling procedure using 4-aminobenzoic acid ethyl ester (benzocaine) and picoline borane as the reducing agent, then applies HPLC separation with UV detection for the analysis of the eight HMOs. Method Scope/Applicability: Method has been applied to the analysis of 8 HMOs (2’-FL, 3-FL, DFL, LNT, LNnT, 6’-SL, 3’-SL, and LNFP-I) in ingredients (premixes) and selected milk protein based infant formula matrices containing maltodextrin. General comments about the method: Document appears to already be largely formatted in alignment with AOAC guidelines. Recommend some additional guidance on establishing equivalence for the reagents listed (purity >x?, impurity

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