AOAC ISPAM Food Allergen WG Meeting Book (12-15-16)

masahiro shoji 2: Recovery range should be changed in spiking case and naturally incured case. Japan uses natually incurred samplen abd range is 50-150%.

Laura Allred: yes, that's why it should be protein, not the allergen. People with allergens can react to different epitopes, but a monoclonal antibody will only ever see one.

France Cho: Agree. Incurred products would give a better picture of what is really recovered in actual products.

Laura Allred: this is where we need a section on method limitations for SMPRs and validations - any method based on one or two protein markers may notwork in fractionated materials.

Laura Allred: this section could also cover the applicability of the method to raw vs cooked material,which is important in egg.

Bert Popping: Would that not exclude methods recently developed by Ghidhari?

Markus L: Whats about reader-based quantitative LFDs?

Bert Popping: I think we need to widen this

Laura Allred: ligand-based assays

Laura Allred: with an appendix specific to ELISA

Hirotoshi Doi: The recovery range should be changed. Spiking case is 80-120%, and incured case is 50- 150% according to Appendex M.

Laura Allred: good point