AOAC ISPAM Meeting eBook, March 17 2015

Salmonella Methods Harmonization Working Group Members

FDA - CFSAN

Thomas Hammack (Chair)

Patrice Arbault

Nexidia

Marcia Armstrong

QIAGEN Gmbh

Mike Clark

Bio-Rad Laboratories Q Laboratories, Inc.

Erin Crowley

Leanne DeWinter Philip Feldsine

Health Canada

BioControl Systems, Inc.

Netherlands Food and Consumer Product Safety Authority

Paul In't Veld Irene Iugovaz

Health Canada

Balamurugan Jagadeesan

Nestec S.A bioMerieux

Ron Johnson Adrianne Klijn

Nestle Quality Assurance Laboratory

W d L

en y auer

Bi R d L b t i o- a a ora or es, nc. I

Wendy McMahon

Silliker Inc.

Sam Mohajer

Canadian Food Inspection Agency

Kirsten Mooijman

Coordinator EURL-Salmonella

Mark Mozola

Neogen Corporation

Brooke Schwartz Meredith Sutzko Morgan Wallace

Brooke Schwartz Consulting

Romer Labs

DuPont Nutrition & Health

Testing for Salmonella

FSIS (USDA) MLG 4.04

FDA BAM Chapter 5

AOACOMA 200.06, 995.20and 967.26

MFHPB 20

ISO 6579

Pre-enrichment in Lactose, Nutrient, UP,

Pre-enrichment in

Pre-enrichment in Lactose or Broth TSB

Pre-enrichment in

Pre-enrichment in BPW or nutrient broth

BPW

or TS broth. Plus others

BPW

Incubation at 35 ºC for 24 hours

Incubation at 35 ºC for 20 to 24 hours

Incubation at 35 ºC for 20 to 24 hours

Incubation at 37 ºC for 16-20 hours.

Incubation at 35 ºC for 18 to 24 hours

Selective enrichment in TT and RV Broth at 35 ºC and 42 ºC and 1 mL and 0.1 mL respectively for 24 h. SC broth at 35˚C for Guar Gum and S. Typhi

Selective enrichment in RV and MKTTn Broth. RVS at 37°C and 42°C and 0.1 mL and 1 mL respectively for 24h

Selective enrichment in TT, RV and / or SC broth depending on method. (1 mL, 0.1 mL and 1 mL respectively) TT and SC are incubated at 35 ºC and RV is incubated at 42 ºC for 24 hours.

Selective enrichment in RVS and TBG broth at 42.5 ºC for 24 h (0.1 mL and 1mL respectively)

Selective enrichment in TTh and mRV, R10, or RVS broth at 42 ºC for 18-24 h (0.5 mL and 0.1 mL respectively)

Streak onto XLD and one other agar (the second agar is any agar for the isolation of salmonella)

Streak onto HE, XLD, BismuthSulfite agar.

BismuthSulfite Agar, HE agar, XLD agar

Streak on at least 2 of the 3: Bismuth Sulfite Agar, BGS, Brilliance Salmonella agar.

Streak onto BGS plus one of DMLIA or XLT 4

Biochemical and serology tests to confirm

Biochemicaland serology tests to confirm

Biochemicaland serology tests to confirm

Biochemical and serology tests to confirm

Biochemicaland serology tests to confirm

Slide Courtesy Donna Douey, Canadian Food Inspection Agency