AOAC ISPAM Meeting eBook, March 17 2015

Virus : detection method?

• Elution • Concentration

CEN/TC275/WG6/TAG4 ISO/TS 15216 PART 1 & 2

PROTOCOL

100 cm 2

2g

QUANTITY PURIFIED VIRAL RNA

25g

1L

From a know volume of concentrate/extraction by standard method

ANALYSIS

Real-time (TaqMan) RT-PCR

Determination

2 PARTS

Qualitative

Quantitative

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Virus : detection method?

CEN/TC275/WG6/TAG4 ISO/TS 15216 Part 1 & 2 PRETREATMENT & EXTRACTION OF NUCLEIC ACIDS SWABBING FOLLOWED BY ELUTION 100c m 2 ELUTION WITH AGITATION & PRECIPITATION WITH PEG / NaCl DIGESTIVE TISSUE PRETREATMENT BY CRUSHING AND PROTEINASE K 2g 25g

ADSORPTION & ELUTION ON LOADED MEMBRANES & CONCENTRATION / ULTRAFILTRATION

1L

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