AOAC ISPAM Meeting eBook, March 17 2015

Draft, Do Not Distribute

Annex II: Inclusivity Panel

74 75 76 77 78 79 80

The inclusivity panel shall include:

• Sequences from at least two representative strains from each major clade

of Variola virus

• Any other strain with differences in the assay primer and/or probe target 81 82 Note: The World Health Organization (WHO) restricts access to Variola virus 83 genomic material; use of any genomic sequences greater than 500 bp requires 84 written permission/approval from the WHO. Insertion of Variola virus DNA into 85 other Orthopoxviruses is prohibited. 88 89 http://www.who.int/csr/disease/smallpox/SummaryrecommendationsMay08.pdf sequences based on bioinformatic analysis (Annex V). 86 87 More details can be found at:

90 91

5 Approved Variola SMPR v7.5