AOAC ISPAM Stakeholder Panel Meeting Book 9-24-17

Table 1 – Spike Data for C Series Samples

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Sample Grain

Cultivar Mass flour (g)

Mass Spike (g)

Calc ppm grain

Gluten spike ppm Dumas water/NaCl

Gluten spike ppm Dumas 0.4 M NaCl in PBS

Gluten spike ppm HPLC

R5 ELISA ppm

B100C B200C

Barley Barley

Jalpur Jalpur

1000 1000 1000 1000 1000 1000

0.1014 101.4 0.2014 201.4 0.1027 102.7 0.201 201.0 0.1022 102.2 0.2019 201.9

5.88

5.67

3.96 7.87 7.83 2.36 4.66 15.33

23.91 39.23 11.17 33.57 16.34 29.86

11.68

11.26

W100C Wheat W200C Wheat

Mix Mix

8.22

7.52

16.08

14.71

R100C R200C

Rye Rye

Holo Holo

3.98 7.87

3.34 6.60

Unspiked

0.958

313 314