AOAC ISPAM Stakeholder Panel Meeting Book 9-24-17

Dose Response Curve to Estimate Recovery

BARLEY: Slope = 29.7%

Standard Method Performance Requirements (SMPRs)

ƒ b The recovery criteria are larger than traditionally used for SMPRs for 2 reasons ƒ (a) Method results for gluten in oats are highly variable due to sample inhomogeneity which may result in a wider range of recovery estimates than would normally be expected at the ppm concentration levels ƒ (b) the different relative specificities of the antibodies against wheat, rye and barley make balancing the response difficult.

Parameter

Acceptance Criteria

Analytical Range (ppm) LOD (ppm) LOQ (ppm) Recovery (%) a

” 5 to

” ”

50 to 200 b Not Defined Yet Not Defined Yet

RSD r

RSD R