AOAC OMA# 2011.06 Final Action Review-OMB

2011.06 (September 2017)-Fol-22 MLT Report

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2.5. Statistical evaluation After data collection, outliers were detected using Cochran and Grubb’s tests. The number and coded identity of statistical outlier laboratories is included in the final report. Average folate concentrations, standard deviations of repeatability (S r ) and relative standard deviations of repeatability (RSD r ) were estimated from blind duplicates in MLT samples. Standard deviations of reproducibility (S R ), relative standard deviations of reproducibility (RSD R ), and HorRat R values (RSD R /predicted RSD R ) were also determined.

3. MATERIALS AND METHODS 3.1 Study Samples :

The study was carried out using SPIFAN matrices, which represent most of the products in the scope of the method (Infant Formula and Adult Nutritionals made from any combination of milk, soy, whey, hydrolyzed protein, starch and amino acids, with and without intact protein). SPIFAN test kit consisting of 11 samples in blind coded duplicates (Table 1) was used for the study. The protocol consisted of analyzing the SPIFAN samples over two separate days as blind coded duplicates. One of the sample was NIST SRM 1869a. The sample details are provided in Table 1. The kit also included two practice samples. The practice samples were run by participants first and if acceptable data was obtained, approval to proceed to analyze the MLT samples was given.

Collaborative Study Report: Method AOAC 2011.06 (Folate by LC-MS/MS) 5