AOAC OMA# 2011.06 Final Action Review-OMB

2011.06 (September 2017)-Fol-22 MLT Report

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Table 4 (continued): Precision results for the AOAC SPIFAN MLT samples

Child formula powder – milk- based

Adult nutritional ready to feed – high fat - Liq

Infant elemental powder

Parameters

Symbol

Units

RQXQ518/ GVPE615

ECHL425/ UOPM297

DYLB360/ ZMQM883

11

11

11

Total number of laboratories

p

22

22

22

Total number of replicates Overall mean of all data (grand mean) Repeatability standard deviation Reproducibility standard deviation

Sum(n(L))

258.6

57.0

349.5

µ g/100g

XBARBAR

14.5

14.3

2.00

µ g/100g

SD r

31.4

31.5

6.1

µ g/100g

SD R

Repeatability relative standard deviation

4.1

5.5

3.5

RSD r

%

9.0

12.2

10.6

Reproducibility relative standard deviation

RSD R

%

0.68

0.61

0.88

HORRAT value

HorRat R

7.0

7.0

7.0

SMPR Repeatability Limit

RSD r

%

16.0

16.0

16.0

SMPR Reproducibility Limit

RSD R

%

0.5-2.0

0.5-2.0

0.5-2.0

HORRAT (Satisfactory Limit) HorRat R

Summary of the method folate repeatability and reproducibility values and Horwitz Ratio for all of the SPIFAN samples are provided in Table 5 and Figures 12, 13 and 14. Precision as repeatability ranged from 3.5-6.6%, RSD r and reproducibility values ranged from 9.0 to 15.7% RSD R . For all SPIFAN samples analyzed in the MLT study were within the limits set in the Folate SMPR (AOAC 2011.006). Acceptable reproducibility was also demonstrated with better than expected HorRat R values for the method ranging from 0.61-1.06 (expected range 0.5-2.0) (4).

Collaborative Study Report: Method AOAC 2011.06 (Folate by LC-MS/MS) 16