AOAC OMA# 2011.06 Final Action Review-OMB

2011.06 (September 2017)-Fol-22 MLT Report

FOR ERP USE ONLY DO NOT DISTRIBUTE

Appendix 1: Method

AOAC First Action 2011.06 – Analysis of Total Folate in Infant Formula and Adult Nutritional Samples by LC-MS/MS A. SCOPE

This method is applicable for determination of total folate in infant, pediatric, and adult nutritionals.

B. SAFETY ALL REAGENTS AND EQUIPMENT SHOULD BE HANDLED IN A MANNER CONSISTENT WITH SAFE LABORATORY PRACTICES. REAGENTS AND SAMPLES SHOULD BE TREATED WITH THE RESPECT AND CARE THAT ANY CHEMICAL REQUIRES. REAGENTS WHICH ARE ALLOWED TO COME IN CONTACT WITH THE SKIN, EYES, OR CLOTHING SHOULD BE FLUSHED IMMEDIATELY WITH LARGE VOLUMES OF WATER. IMMEDIATE MEDICAL ATTENTION SHOULD BE OBTAINED IF ANY REAGENT IS INGESTED OR ALLOWED TO COME IN CONTACT WITH THE EYES. METHANOL USED IN THIS METHOD IS HIGHLY FLAMMABLE AND SHOULD BE KEPT AWAY FROM FLAMES AND OTHER IGNITION SOURCES METHANOL IS A TOXIC CUMULATIVE POISON THAT CAN CAUSE BLINDNESS. ACETIC ACID AND FROMIC ACDI ARE STRONG OXIDIZER. DIRECT CONTACT MAY CAUSE IRRITATION TO SKIN AND EYES. Caution: Refer to Material Safely Data Sheets (MSDS) of all chemicals prior to use and follow the suggested personal protective equipment and proper disposal procedures. C. THEORY 1. Folate is a group of water-soluble vitamers. Pteroic acid is the parent compound. Folate refers to a group of compounds structurally based on pteroic acid conjugated with one or more L- glutamate linked through the γ-carboxy group of the amino acid. Folic acid contains one glutamic acid residue and know as pteroyl glutamic acid. 2. Folate structure differs by the reduction and substitution on the pterin group, and also by the length of the glutamate chain (Figure 1).

Collaborative Study Report: Method AOAC 2011.06 (Folate by LC-MS/MS) 22