AOAC OMA# 2011.06 Final Action Review-OMB

2011.06 (September 2017)-Fol-22 MLT Report

FOR ERP USE ONLY DO NOT DISTRIBUTE

3. Folic acid is not a natural substance but it is the common and stable form of the vitamin used in food fortification and formulation and in dietary supplements and pharmaceuticals. 4. Good dietary sources of native folate include liver, legumes, green leafy vegetables, citrus fruits and juices, certain berries, cereals, and egg . Enriched cereal products are often fortified with folic acid. Infant and pediatric formulas and adult nutritionals are often fortified with folic acid. The described method is a LC-MS/MS method for determination of total folate,based on analysis of important folate vitamers in various types of infant formula and adult nutritional samples after trienzyme digestion. The chromatographic method is capable of determining various folate vitamers including folic acid, 5-methyl-THF and 5-formyl-THF in infant, adult, and pediatric nutritional powders, ready-to-feed liquids, and liquid concentrates and was developed to meet the Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) method performance requirements.

C: FLOW DIAGRAM:

Collaborative Study Report: Method AOAC 2011.06 (Folate by LC-MS/MS) 23