AOAC OMA# 2011.14 (Final Action Review)-OMB

SPIFAN Expert Review Panel Report September 29, 2015 Final Version

Chlor-03 -Single Laboratory Validation for Chloride Analysis in Infant Formula and Adult Nutritionals: AOAC SMPR 2014.015

Bill Mindak Günther Raffler Karen Schimpf

Method not adopted/ moved to First Action Official Method SM status

 Dilution factor  general ion chromatography to do chloride  method is sophisticated  Contains high bias on the reference material  Method does not meet some of the SMPR  Lower linear limit did not meet SMPR  Write method more neutral/general  Need report/data  Failed to meet SMPR  Needs additional work done  Method did not meet the SRM certified reference range  Need additional information on true value w/NIST

Chloride

Fluor-02 - Single Laboratory Validation for Fluoride Analysis in Infant Formula and Adult Nutritionals: AOAC SMPR 2014.016 Fos-04 - Determination of Fructans in Infant and Adult/Pediatric Nutritional Formulas as well as ingredient commodities

John Austad Melissa Phillips Bill Mindak

Method not adopted/ moved to First Action Official Method SM status

Fluoride

 Data will meet SMPR  Spiked recovery

Jon DeVries Sean Austin Hans Cruijsen

Jon DeVries (moved) Brendon Gill (second) Yes-4/ No-10/Abstain-1 _________________ Method not adopted/ moved to First Action Official Method SM status Maria Ofitserova (moved) Estela Kneeteman (second) Yes-2/ No-13/Abstain-1 _________________ Method not adopted/ moved to First Action Official Method SM status

Fructans (FOS)

o Part 1 - oligosaccharide (need to see data)  The units used in the report are not clearly described

 Method not easy to run (part 1 & 2)  No blank samples (analyze all SPIFAN samples)  Method does not meet SMPR  Four (4) spiked levels w/ two (2) below  Higher GOS levels got worst  Meets repeatability requirement  Need more data/reference  Method doesn't capture DP2  Uses commonly available equipment  Removes Lactose to improve accuracy of GOS analysis  SPIFAN matrices were used for Single Laboratory Validation Study  Method has a simple microwave digestion  No ionization buffer is required o should be required & stated o do background correction  Specify the wavelength  LOQ for three (3) elements o copper o iron o manganese  No data on SPIFAN matrices  Method is easy to follow  Method is not clearly written  Recovery rates were combined  Free forms are in range  The scope of the method does not meet the SMPR  Applicability does not meet SMPR  Does not capture phosphate

Gos-02 - Determination of trans-Galacto- oligosaccharides (TGOS) in Infant Milk Formula (Ion- Exchange Chromatography)

Sean Austin Maria Ofitserova Estela Kneeteman

GOS

MTE-03 - ISO/CD 15151/IDF 229 Milk and milk products- Determination of calcium, copper, iron , magnesium, manganese, phosphorus, potassium, sodium and zinc contents. Inductive coupled plasma atomic emission spectrometric method (ICP- AES) BVit-01 - Simultaneous Determination of Thiamine HCl, Riboflavin, Niacin (Nicotinic Acid and Nicotinamide), Pantothenic acid, Vitamin B6 (Pyridoxine, Pyridoxal and Pyridoxamine) and Biotin in Infant Formula and Adult Nutritionals

Bill Mindak Jinchuan Yang

Jinchuan Yang (moved) Scott Christiansen (second) Yes-6/ No-7/Abstain-1 _________________ Method not adopted/ moved to First Action Official Method SM status

Minerals & Trace Elements

Esther Campos- Gimenez Brendon Gill Scott Christiansen

Method not adopted/ moved to First Action Official Method SM status

B Vitamins (1, 2, 3, 6)

 FAD/FAB

BVit-02 - Simultaneous Determination of Total

Esther Campos- Gimenez Scott Christiansen Estela Kneeteman

Scott Christainsen (moved) Sarwar Gilani (second) Yes-6/ No-9/Abstain-1 _________________ Melissa Phillips (moved) Esther Campos-Giminez (second) Yes-11/ No-2/Abstain-1

o FAB is missing  Use of 1849a is not needed and should not compare

Vitamin B6, B2, B3 and B1 in Infant Formula Products by LC-MS/MS Using Enzymatic Digestion

 ERP wants to see blanks  Niacin in most is lower

Yes-12/ No-2/Abstain-1

**Method adopted/ moved to First Action Official Method SM status for B 1 , 2 , 6 only

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