AOAC OMA# 2017.03 (Final Action)-OMB

2017.03 (JULY 2018) Amino-02 Tryptophan by enz hyd_Abbott Labs_Method

FOR ERP USE ONLY DO NOT DISTRIBUTE

HPLC Determination of Total Tryptophan in Infant Formula and Adult/Pediatric Nutritional Formula Following Enzymatic Hydrolysis

Jonathan Draher and Norman White Abbott Nutrition, Division of Abbott Laboratories

This method is applicable tofor the determination of total tryptophan in infant formula and adult/pediatric nutritional formulas. A sample amount targeting aboutapproximately 30 – 50 mg of protein is added to the sample preparation. The limit of quantitation of a typical ready to feed formula is approximately 0.18 mg/100g. A. SAFETY ALL REAGENTS AND EQUIPMENT SHOULD BE HANDLED IN A MANNER CONSISTENT WITH SAFE LABORATORY PRACTICES. REAGENTS AND SAMPLES SHOULD BE TREATED WITH THE RESPECT AND CARE THAT ANY CHEMICAL REQUIRES. REAGENTS WHICH ARE ALLOWED TO COME IN CONTACT WITH THE SKIN, EYES, OR CLOTHING SHOULD BE FLUSHED IMMEDIATELY WITH LARGE VOLUMES OF WATER. IMMEDIATE MEDICAL ATTENTION SHOULD BE OBTAINED IF ANY REAGENT IS INGESTED OR ALLOWED TO COME IN CONTACT WITH THE EYES. ALL OF THE SOLVENTS USED IN THIS METHOD ARE HIGHLY FLAMMABLE AND SHOULD BE KEPT AWAY FROM FLAMES AND OTHER IGNITION SOURCES. METHANOL IS A TOXIC CUMULATIVE POISON THAT CAN CAUSE BLINDNESS. PHOSPHORIC ACID AND HYDROCHLORIC ACID MAY CAUSE IRRITATION TO SKIN AND EYES IF DIRECT CONTACT IS MADE. USE CARE WHEN HANDLING STREPTOMYCES GRISEUS ENZYME: MAY BE HARMFUL IF INHALED. MAY CAUSE RESPIRATORY TRACT, EYE AND SKIN IRRITATION. MAY CAUSE ALLERGIC RESPIRATORY AND SKIN REACTION.

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