AOAC OMA 2021.01 Reviewer Forms (July 25, 2023)

FOR EXPERT REVIEW PANEL USE ONLY NOT FOR DISTRIBUTION (DRM)

Expert Review Panel for Infant Formula and Adult Nutrition “FINAL ACTION REVIEW” Evaluation of Method 2021.01 (GOS-03)

Title: Determination β -Galactooligosaccharides (GOS) in Infant Formula and Adult Nutritionals: Collaborative Study Author: Denys Cuany, Sean Austin Reviewer Name: Reviewer 1

Did Method Author Consider ERP’s General Recommendations:

• Was the method tested using all the SPIFAN samples for precision and accuracy? The method was tested using MLT sample kit comprised of seven commercial products from different suppliers. Six samples of Infant Formula with with different composition GOS/FOS, GOS/HMO, partially hydrolyzed protein, organic with probiotics and one sample of Adult Nutritional.

• Was data provided from a standard reference material? Yes, data was provided by NIST SRM 1849b.

• Was the analysis conducted using ready-to-feed (reconstituted) infant formula? Yes, three ready-to-feed infant formula samples were analyzed.

• Was rice-based formula available and was it used? No rice-based formula available.

• Was the method tested using properly characterized standards? Yes, the method was tested using laminaritriose as internal standard and with maltotriose as external standard.

• Is there proof of performance through system suitability? Yes, the system suitability is described in the collaborative study as system suitability test (item I) • Is there clarity of calibration procedure? Yes, it is recommended to use bracketed calibration, injecting 3 standards followed by a maximum of 10 samples then 3 standards again. It is used the instrument software to plot a six points standard

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