AOAC OMB Final Action Recommendation (December 2019)-2016.14
FOS-03 FOR WORKING GROUP/ERP USE ONLY DO NOT DISTRIBUTE
(q) Acetic acid, glacial 100% anhydrous, GR for analysis
(r) Trifluoroacetic acid
(s) D-(-)-Fructose, Biochemika, >99%
(t) Acetonitrile
(u) Sodium maleate buffer solution (100 mM, pH 6.5) - Into a large beaker (>1000 mL)
weigh 11.6 g of maleic acid (puriss., >99%) and dissolve with 900 mL of water. Adjust
the pH to 6.5 with 1 M sodium hydroxide solution (p). Transfer the solution to a
1000 mL volumetric flask and make up to the mark with water.
(v) Sodium acetate buffer solution (100 mM, pH 4.5) - Into a large beaker (>1000 mL)
containing 900 mL of water, pipette 5.8 mL of glacial acetic acid (q). Adjust to pH 4.5
with 1 M sodium hydroxide solution (p). Transfer the solution to a 1000 mL
volumetric flask and make up to the mark with water.
(w) Sodium hydroxide solution (50 mM) - Into a 100 mL volumetric flask, weigh 0.2 g of
sodium hydroxide pellets and dissolve with 90 mL of water. Make up to the mark
with water.
(x) Sodium borohydride (10 mg/mL in 50 mM sodium hydroxide) – accurately weigh 50
mg of sodium borohydride in to a polypropylene tube and record the weight.
Immediately before use dissolve in an appropriate volume of 50 mM sodium
hydroxide solution (w) to give a final concentration of 10 mg/mL.
(y) Acetic Acid (0.2 M) – Into a 1000 mL volumetric flask containing 900 mL of water,
pipette 11.6 mL of glacial acetic acid (q). Make up to the mark with water.
(z) Fructan Assay Kit, K-FRUC (Megazyme International Ireland Ltd, Wicklow, Ireland).
(aa) Sucrase (~4.5 U/mL) / α-amylase (~23 U/mL) / pullulanase (~4.5 U/mL) / maltase
(~45 U/mL) solution; dissolve the contents of the sucrase / amylase /pullulanase /
maltase vial from the fructan assay kit (z) in sodium maleate buffer (m) following the
instructions from the kit. Divide into aliquots of 2.0 mL (enough for 9 sample
determinations) and store frozen at -20°C in polypropylene tubes until required.
(bb)
Fructanase solution (exo-inulinase ~ 455 U/mL + endo-inulinase ~4.5 U/mL);
dissolve the contents of the fructanase vial from fructan assay kit (z) in sodium
acetate solution (v) following the instructions from the kit . Divide into aliquots of
2.0 mL (enough for 18 sample determinations) and store frozen at -20°C in
polypropylene tubes until required.
(cc) SPE wash solution - Into a 100 mL flask, add 80 mL of acetonitrile and 100 µL of TFA.
Make up to the mark with water.
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