AOAC-RI ERP Book - FAOM METHOD.pdf

AOAC RESEARCH INSTITUTE AOAC OFFICIAL METHODS OF ANALYSIS (OMA) OMAMAN-15 : Determination Of Polycyclic Aromatic Hydrocarbons (PAHs) In Seafood Using Gas Chromatography-Mass Spectrometry: A Collaborative Study* Study Director: Katerina Mastovska, Covance Laboratories Inc., Nutritional Chemistry and Food safety, 3301 Kinsman Boulevard, Madison, WI 53704, USA

Precision

ER 1

Varies. Mostly around or below 10%, with one exception of 27% for low level 1-MN.

ER 2 Precision and reproducibility varies and is dependent on the specific analyte. The reported values meet the validation criteria ER 3 Good ER 4 In Shrimp: 1.40-26.9% In mussel: 2.52-17.1% In oyster: 3.12-22.7% ER 5 Good ER 6 I am not familiar with the expectations for precision for an AOAC method; however, the precision here appears to be adequate. ER 7 Precision was excellent. ER 8 good ER 2 Precision and reproducibility varies and is dependent on the specific analyte. The reported values meet the validation criteria ER 3 Good ER 4 In Shrimp: 5.41-29.4% In mussel: 4.19-32.5% In oyster: 8.41-31.8% ER 5 Good ER 6 I am not familiar with the expectations for reproducibility for AOAC method; however, the reproducibility for this study appears to be inadequate for many of the more volativle PAHs (e.g., naphthalene) and particularly in the oyster tissue. ER 7 Reproducibility was good except for a few compounds in oyster stored at -20 C. ER 8 good Reproducibility ER 1 Varies. Mostly between 10%-20%.

System Suitability Not discussed. System is suitable

ER 1 ER 2 ER 3 ER 4

Good

na

ER 5 Were IDLs, MDLs and PQLs carried out on all instrumentation used in the Collaborative Study? If not, this should be performed and documented in the Method. Are there records of Intra-day, Inter-day variability? Are there records of Analyst variability? If so, the Method should state. ER 6 No comments ER 7 System check samples were analyzed. ER 8 very good