AOAC-RI ERP Book - FAOM METHOD.pdf

AOAC RESEARCH INSTITUTE AOAC OFFICIAL METHODS OF ANALYSIS (OMA) OMAMAN-15 : Determination Of Polycyclic Aromatic Hydrocarbons (PAHs) In Seafood Using Gas Chromatography-Mass Spectrometry: A Collaborative Study* Study Director: Katerina Mastovska, Covance Laboratories Inc., Nutritional Chemistry and Food safety, 3301 Kinsman Boulevard, Madison, WI 53704, USA

First Action Recommendation

ER 1 ER 2 ER 3

Yes. Yes Yes

ER 4 I recommend that the method, which has been gone through AOAC collaborative study successfully, for determination of PAHs in seafood using GC-MS be adopted Official Fist Action ER 5 No ER 6 Not yet....I think it needs validation with a natural matrix CRM such as SRM 1974c. ER 7 Yes, with minor modifications (please see After First Action Recommendation) ER 8 yes

After First Action Recommendation

ER 1 ER 2 ER 3 ER 4

Explore for ways to improve inter-lab precision RSD(R)%

NO

See comments above.

na

ER 5 It may be helpful to refer to the FDA's LIB # 4475 to get a better feel for how the Method should be formatted and important quantitative data to include. The only exception here is the Safety Section is not in the front of this FDA LIB. ER 6 As mentioned above, information on the method performance using SRM 1974c ER 7 Fish samples should be analyzed in the future to see if this method is applicable to fish as well, especially those with high fat content. Was matrix effect significant? or the internal standards (13C PAHs) added to samples before extraction corrected the matrix effect of their corresponding PAHs? How about the alkyl PAHs that did not include their isotope labeled standards in this study? Should matrix matched calibration be more appropriate? I would suggest the study group to compare the PAH recoveries using this method and one of the other currently accepted methods to test an oyster reference material stored at - 20 C to show if the degradation of Ant and BaP is method dependent. ER 8 no